摘要
Aim To construct an efficient recombinant viral vector for gene therapy.Methods(First-generation) adenovirus(Ad) vector was modified with the RGD peptide inserted into the fiber.Both in vitro and in vivo experiments of gene expression in different tumor cells with conventional and recombinant vectors were conducted.RT-PCR was used for detecting the expression of coxackievirus and adenovirus receptor and integrin at the surface of Meth-A cells.Results Fiber-mutant adenovirus vector showed a notably enhanced gene expression in A2058,B16BL6,OV-HM,and Meth-A tumor cells compared with that of conventional ones.In vivo study carried out using Meth-A tumor-bearing mice also demonstrated that the intra-tumoral injection of recombinant adenovirus induced strong gene expression in these CAR-deficient tumor cells. Conclusion The recombinant vector can be a promising one for effective cancer gene therapy.
Aim To construct an efficient recombinant viral vector for gene therapy. Methods First-generation adenovirus （Ad） vector was modified with the RGD peptide inserted into the fiber. Both in vitro and in vivo experiments of gene expression in different tumor cells with conventional and recombinant vectors were conducted. RT-PCR was used for detecting the expression of coxackievirus and adenovirus receptor and integrin at the surface of Meth-A cells. Results Fiber-mutant adenovirus vector showed a notably enhanced gene expression in A2058, B16BL6, OV-HM, and Meth-A tumor cells compared with that of conventional ones. In vivo study carried out using Meth-A tumor-bearing mice also demonstrated that the intra-tumoral injection of recombinant adenovirus induced strong gene expression in these CAR-deficient tumor cells. Conclusion The recombinant vector can be a promising one for effective cancer gene therapy.
出处
《药学学报》
CAS
CSCD
北大核心
2006年第11期1116-1120,共5页
Acta Pharmaceutica Sinica
基金
国家自然科学基金资助项目(30572270)
关键词
重组腺病毒
肿瘤细胞
基因表达
整合素
recombinant adenovirus vector
tumor cell
gene expression
integrin
