体外诱导培养人树突状细胞的生物学特性及其神经免疫调节功能分析

Analyses on Biological Characteristics and Neuroimmuno-modulatory Functions of Epidermal Dendritic Cells Induced and Cultured in Vitro

目的研究人表皮树突状细胞在皮肤的免疫反应和疾病发生发展过程中的作用。方法取胎儿脐带血分选单核细胞,在体外用粒-巨噬细胞集落刺激因子(GM-CSF)和白介素-4(IL-4)进行定向诱导分化,培养树突状细胞(MoDC),观察培养细胞在不同阶段的变化;用流式细胞仪分析诱导分化细胞的细胞膜表面标志性抗原HLA-DR、CD1 a和CD80的表达;对诱导分化的细胞分别给予脂多糖体(LPS),LPS和降钙素相关基因肽(CGRF)和单纯CGRF刺激,检测不同处理后MoDC表达IL-12 mRNA变化。结果脐带血干细胞在体外诱导分化培养第7天呈现明显树突状分支,并可检测到HLA-DR和CD1 a分子的表达。结论CGRP对MoDC表达IL-12 mRNA的调节有双重性。

Objective To study the effect of epidermal dendritic cells on the immune reaction and disease development in the skin. Methods The cells were taken from embryo umbilical blood and divided into monocytes. The monocytes were cultured with GM CSF and IL-4 in vitro and investigated the changes in different stage. The MoDC were analyzed the HLA-DR, CDla and CD80 antigen markers on the cell surface with FACS. The MoDC were stimulated with LPS, CGRP and LPS, simple CGRP, then the expression of IL- 12 mRNA in the MoDC were tested. Results The monocytes from umbilical blood appeared dendritic cells in culture 7 days later and expressed HLA-DR, CDla marker. Conclusion The neuropeptide CGRP played double modulation on the MoDC in expression of IL-12 mRNA.