APP17肽对长波紫外线辐射人皮肤成纤维细胞氧化损伤的保护机制

Study on the Protection Mechanism of APP17-mer Peptide from Ultraviolet a Induced Damage on Cultured Fibroblasts

目的探讨β-淀粉样蛋白前体蛋白17肽(-βmyloid precursor prote in 17-er peptide,APP 17)对长波紫外线(UVA)辐射后人皮肤成纤维细胞的保护作用及其机制。方法用UVA照射培养人皮肤成纤维细胞,四甲基偶氮唑盐比色法(MTT)检测细胞活性,激光共聚焦显微镜检测活性氧(ROS)的产生,生化法检测总超氧化物歧化酶(SOD)的活性。结果UVA辐射后成纤维细胞活性降低(P=0.000),细胞内ROS增多(P=0.000),总SOD活性增加(P=0.01)。40μmol/L的APP17肽能拮抗UVA辐射对成纤维细胞的影响(P<0.05)。结论APP17肽对UVA辐射损伤的成纤维细胞有保护作用。

Objective To investigate the protection mechanism of β-amyloid precursor protein 17-mer peptide from UVA induced damage on cultured fibroblasts. Methods Human skin fibroblasts were irradiated with UVA. The activity of fibroblast was detected with the assay of MTT. The activity of superoxide dismutase was measured with biochemical assay. The intracellular ROS production was measured with a confocal microscopy. Results A significant decrease of activity of fibroblast was observed after exposure to UVA （ P = 0. 000）. A higher level of intracellular ROS（ P = 0. 000）and activity of superoxide dismutase（ P = 0.01 ） were detected in the irradiated samples. 40 μmol/L APP17-mer peptide could counteract the effects of UVA on fibroblasts （ P 〈 0.05 ）. Conclusion APP 17-mer peptide has potential protective effects from UVA induced damage on cultured fibroblasts.