不同细胞株建立人前列腺癌裸小鼠移植模型及转移特性比较

Establishing Human Prostate Cancer Models from Different Cells and Its Metastatic Character

目的比较人前列腺癌细胞DU145和CWR22Rv1(22Rv1)(下面简称22Rv1)分别在皮下和前列腺原位接种后的成瘤性和转移率。方法采用原位接种及皮下接种两种方法将前列腺癌细胞DU145和22Rv1分别接种于裸小鼠体内,以裸鼠出现恶液质、处于濒死状态作为观察终点,颈椎脱臼法处死。尸解并观察荷瘤部位肿瘤的生长情况及局部淋巴结自发性转移情况,并取皮下肿瘤、原位肿瘤、肝、肺、肾、膀胱、盆腔淋巴结标本,甲醛固定、石蜡包埋和HE染色后显微镜下观察。结果原位接种22Rv1组的裸小鼠成瘤率达到10/10,淋巴结转移率为9/10；原位接种DU145组的裸小鼠成瘤率达到10/10,而淋巴结转移率为1/10。两种细胞皮下接种法的裸小鼠成瘤率均达10/10,但未检测到任何淋巴结转移。结论对这两种细胞株采用原位接种法建立裸鼠人前列腺癌原位模型获得成功,用22Rv1细胞建立的原位模型淋巴结转移率高于DU145组,为前列腺癌转移机制的研究及筛药提供了模型。

Objective To compare the biological character between the model of human prostate cancer cell DU145 and 22Rvl utilizing ectopic and orthotopic implantation in nude mice. Methods The human prostate cancer cell DU 145 and 22Rv1 were inoculated under orthotopic or ectopic environments separately in nude mice, the mice were were sacrificed at the agonal stage. The tumorigenicity and lymph node metastasis were determined by gross and histological examinations. Results The 22Rv1 cells were equally tumorigenic with 10/10 frequency in the prostate and subcutis, they exhibited highly metastasis potentiality with 9/10 lymph node metastasis after orthotopic implantation in nude mice for 40 days, whereas no lymph node metastasis after ectopic implantation. The DU145 cells were equal tumorigenic with 10/10 frequency in the prostate and subcutis, they exhibited lowly metastasis potential- ity with 1/10 lymph node metastasis after orthotopic implantation in nude mice, and no lymph node metastasis after ectopic implantation. Conclusion The lymph node metastasis model established by orthotopic implantation in nude mice is successful, and it may become a valuable model for the study of metastasis mechanism and experimental therapy of prostate cancer.