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电子射线局部辐照大鼠Ⅰ/Ⅲ型胶原表达及基质金属蛋白酶改变的研究 被引量：1

Study on Changes of Collagen and MMPs after Electron Irradiation in Rats
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摘要 目的探讨电子射线(模拟β射线)局部辐照大鼠致放射损伤后胶原表达及相关基质金属蛋白酶(MMPs)变化的情况。方法采用直线加速器局部照射制作SD大鼠的辐射损伤模型,观察Ⅰ/Ⅲ型胶原表达及MMP1、MMP2、MMP9表达的变化情况。结果β射线照射引起的皮肤损伤可以引起胶原含量和分型的改变,MMP-1有不同程度的提高,MMP2与MMP9的表达随辐射剂量的增加,由强变弱再上升变强。结论β射线照射皮肤损伤引起的创面难愈合可能与胶原含量和结构的改变相关,其又与胶原的降解酶MMP1活性增强有关；MMP2与MMP9参加基底膜的溶解、血管形成和坏死组织的清除,有助于创面的重建,促进创面的愈合。 Objective To investigate the changes of collagen and MMPs after β irradiation mimicked by electrons-ray. Methods An animal model of β irradiation injury was established by using linear accelerator electron radiation. The expressions of collagen and MMP1, MMP2, MMP9 were measured. Results After electrons irradiation, the expression and the structure of collagen were changed. MMP1 was increased. The expression of MMP2 and MMP9 were also changed. At first they were decreased and then increased. Conclusion Difficult healing of the wound caused by β irradiation may have a relationship with the collagen change, which may be connected with the activity change of MMP1. At the same time MMP2 and MMP9 can help the wound healing.
作者 周迎会 仇灏 徐岚 姜智 孙其昌 马震宇 吴士良
机构地区 苏州大学医学院生化与分子生物学教研室暨苏州大学生化工程研究所
出处 《实验动物与比较医学》 CAS 2006年第4期227-230,共4页 Laboratory Animal and Comparative Medicine
基金 国防科工委国防基础科研项目(K0102061501) 江苏省高校自然科学研究基金课题资助项目(04KJB320129) 苏州大学医学发展基金(2007)
关键词 电子射线 放射损伤 胶原表达 基质金属蛋白酶 Electrons-ray Radiation injury Collagens expression MMPs
分类号 Q691 [生物学—生物物理学]
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参考文献14

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二级参考文献9

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实验动物与比较医学
2006年 第4期

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