冠心宁片对老年大鼠血液流变学的影响

Effect of Guanxinning Tablet on Hemorrheology in Aged Rats

目的观察冠心宁片对老年大鼠的血液流变学和相关生化指标的影响。方法取15月龄老年雄性SD大鼠50只,随机分成老年对照组、低(0．3 g/kg)、中(0．6 g/kg)、高(1．2 g/kg)冠心宁片组、阳性对照组各10只,另取3月龄成年雄性SD大鼠10只作正常对照组,各组每日给予相应药物,连续15d后,测定血液流变学和血清葡萄糖(GLU)、总胆固醇(TC)、甘油三酯(TG)、超氧化物歧化酶(SOD)和丙二醛(MDA)等指标。结果给予高、中、低剂量的冠心宁片均可有效降低老年大鼠低切、中切、高切三种状态下的全血粘度及还原全血粘度,显著降低红细胞刚性指数,显著提高中老年大鼠血清SOD,降低MDA含量。高剂量的冠心宁片还具有显著降低TC和GLU的作用。结论冠心宁片对老年大鼠的血液粘滞性和红细胞流变性的异常有显著的改善作用,并可抑制老年大鼠自由基的脂质过氧化,保护抗氧化酶的活性,增强机体自由基的清除,具有活血化瘀的作用。

Objective To observe the effect of Guanxinning tablet on hemorrheology and correlative biochemical indexes in aged rats. Methods Fifty 15-month-old male SD rats were randomly divided into 5 groups including a model control group, a positive control group and low （0.3 g · kg^-1）, middle （0.6g · kg^-1） and high （1.2 g · kg^-1） dose groups of Guanxinning tablet. An additional ten 3-month-old rats were used as the normal control group. Determine the hemorrheology and the biochemical indexes of glucose （GLU）, total cholesterol （TC）, triglyeride （TG）, superoxide dismutase （SOD） and malondialdehyde （MDA） in blood serum of rats after 15 days. Results Guanxinning tablet with low, middle and high dose had significant depressed effect on the level of blood viscosity, plasma viscosity, erythrocyte rigidity index, and significant elevated effect on the activity of SOD and depressed effect on the content of MDA in serum of aged rats. Guanxinning tablet with high dose had significant depressed effect on the level of TC and GLU. Conclusion Guanxinning tablet had significant amendatory effect on the abnormity of blood viscosity and erythrocyte rheological characteristic in aged rats, and contributed to inhibit the peroxidation of free radical, protect the activity of anti-oxidative enzyme, boost elimination ability for free radical. In a word, Guanxinning tablet had the characteristic of promoting blood circulation by removing blood stasis.