PCR同时检测变形链球菌和远缘链球菌

Simultanous Detection of Streptococcus Mutans and Streptococcus Sobrinus by Polymerase Chain Reaction

目的:建立一种快速、简便地同时检测变形链球菌和远缘链球菌的方法。方法:以变形链球菌Dextranase基因设计引物,用PCR检测变形链球菌群细菌。结果:变形链球菌(血清型c、e、f)的PCR扩增产物为720 bp;远缘链球菌(血清型d、g)的PCR扩增产物为460 bp;道勒链球菌(血清型h)910 bp;仓鼠链球菌(血清型a),鼠链球菌(血清型b)均为1 270 bp。其它异种菌均不能扩增出产物,因此该PCR检测具有高度的特异性。从细菌纯培养物中PCR检测的敏感性为105菌落形成单位(colony-form ing un its,CFU)。结论:PCR能同时检测变形链球菌和远缘链球菌。该检测方法具有较高的敏感性和特异性,有望运用于临床检测。

Objective： To establish a simple and rapid method to detect Streptococcus mutans and Streptococcus sobrinus simultaneously. Methods： Chromosomal DNA from the bacteria was obtained by extracted with phenol -chloroform. PCR method with oligonucleotide primers specific for portions of Dextranase genes of S . mutans was optimized to detect S. mutans and S. sobrinus. Results： After PCR, S. mutans and S. sobrinus were specifically identified. PCR was capable of amplifying DNA fragments specific to these species from chromosomal DNA extracted from 105 colony - forming units cells. Conclusion： PCR could detect S. mutans and S. sobrinus rapidly and simutaneously.