人骨髓间充质干细胞培养方法

The Culture of Human Bone Marrow Mesenchymal Stem Cells

骨髓间充质干细胞(BMMSCs)是一种多潜能的成体干细胞,在细胞治疗和组织工程上具有广阔的应用前景。对供体年龄、分离方法、培养密度、培养基和培养基质表面性质对细胞增殖的影响进行了比较,重点阐述了用人自体血清结合多种细胞因子,替代胎牛血清培养BMMSCs的效果,转染端粒酶基因的BMMSCs的增殖能力和分化潜能,以及灌注培养反应器用于大规模培养的技术进展。

Bone marrow mesenchymal stem cells （BMMSCs） are a kind of adult stem cells with multiple potential functions in cell proliferation and differentiation and will be wildly used for cell therapy and tissue engineering in the future. Many factors are reviewed in this paper which include donors＇ age, isolation methods, cell culture density, surface materials and their effects on cell proliferation. It is emphasized that the development of different substitution, such as growth factors and human autologous serum, for fetal bovine serum （FBS） to avoid the risk from some viruses. The BMMSCs introduced by the plasmid with telomerase gene have shown stronger proliferation ability and multiple differentiation potential. The development of perfusion culture systems will make the preparation of BMMSCs more suitable for clinical application.