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洋葱细胞质雄性不育系101A的分子鉴定 被引量：8

Molecular Identification of Cytoplasmic Male Sterility Line 101A of Onion
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摘要 根据文献报道设计引物序列,对洋葱细胞质雄性不育系101A进行PCR扩增,结果表明:洋葱细胞质雄性不育系101A属于S型细胞质雄性不育类型,其特异片段位于叶绿体基因组。 The primer sequence was designed according to the literatures and the cytoplasmic male sterility line 101A of onion was amplified by PCR;It was shown that cytoplasmic male sterility line 101A was found to belong to S type of cytoplasmic male sterility and its specific band was located in chloroplast genome.
作者 陈沁滨 侯喜林 王建军 冷月强 蒋芳玲 薛萍
机构地区 南京农业大学作物遗传与种质创新国家重点实验室 连云港市农业科学院
出处 《西北植物学报》 CAS CSCD 北大核心 2006年第12期2430-2433,共4页 Acta Botanica Boreali-Occidentalia Sinica
基金 江苏省农业科技攻关项目(BE2001319) 连云港市科技攻关项目(NY0403)
关键词 洋葱 细胞质雄性不育 PCR onion cytoplasmic male sterility PCR
分类号 Q343.34 [生物学—遗传学]
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参考文献6

  • 1王建军,侯喜林,宋慧,王学文.洋葱育种研究进展[J].中国蔬菜,2003(4):57-59. 被引量：29
  • 2WANGYH(王彦华) HOUXL(侯喜林) XUMY(徐明宇).Optimalization of ISSR reaction system in non—heading Chinese cabbage by orthogonal design[J].西北植物学报,2004,26(1):899-901.
  • 3ENGELKE T,TEREFE D,TATLIOGLU T.A PCR-based marker system monitoring CMS-(S),CMS-(T) and (N)-cytoplasm in the onion (Allium cepa L.)[J].Theor.Appl.Genet,2003,107:162-167.
  • 4J.萨姆布鲁克,E.F.弗里奇,T.曼尼阿蒂斯.分子克隆实验指南2版[M].金冬雁,黎孟枫等,译.北京科学出版社,1998:19-55.
  • 5SATO Y.PCR amplification of CMS-specific mitochondrial nucleotide sequences to identify cytoplasmic genotypes of onion (Allium cepa L.)[J].Theor.Appl.Genet.,1998,96:367-370.
  • 6赵军良,梁爱华.高等植物细胞质雄性不育分子机理的研究进展[J].西北植物学报,2004,24(8):1543-1546. 被引量：12

二级参考文献26

  • 1Khrustaleva L I, Kik C [a]. Introgression of Allium fistulosum into A.cepa mediated by A. roylei. Theoretical & Applied Genetics. 2000,100(1) :17 ~16.
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共引文献39

同被引文献132

引证文献8

二级引证文献29

西北植物学报
2006年 第12期

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