慢性乙型肝炎患者外周血CD8^+/CD28^+淋巴细胞及其亚型的研究

CD8^+/CD28^+ lymphocyte and their subtypes in the peripheral blood of patients with chronic hepatitis B

目的探讨慢性乙型肝炎患者外周血CD8+/CD28+淋巴细胞及其亚型与其临床状态和HBV复制的关系。方法采用流式细胞技术多色荧光分析法,检测研究对象的外周血CD8+淋巴细胞、CD45RO、CD45RA以及CD28的表达及HBV病毒载量。结果①慢性HBV携带组CD8+淋巴细胞CD28的表达率(10.64±5.09%)明显低于慢性乙肝组和正常对照组;而CD45RA和CD45RO的表达差异无统计学意义;②慢性乙肝组CD8+/CD45RO+/CD28+的表达(10.99±7.33%)明显高于正常对照组,而慢性乙肝组和慢性HBV携带组CD8+/CD45RA+/CD28+表达率均明显低于正常对照组;③HBeAg阴性慢性乙肝CD8+/CD45RO+/CD28+表达率明显高于阳性组,而CD8+/CD45RA+/CD28+表达率两组无差别。结论慢性HBV携带者处于病毒携带状态可能与CD8细胞上协同分子CD28的表达低下有关;CD8+/CD45RO+/CD28+亚型淋巴细胞数的升高与慢性乙肝的病情进展及血清HBeAg转换有关。

Objective To explore the relationship between the the CD8^＋/CD28 ^＋ lymphocyte and their subtypes in the peripheral blood and the clinical condition or the HBV replication of chronic hepatitis B. Methods The CD45R0,CD45RA and CD28 of peripheral blood CD8^＋lymphocyte were detected by flow cytometry with multicolor fluorescence technology in 280 chronic hepatitis B cases, 28 HBV asymptomatic carriers （ASC） and 22 healthy volunteers. Results （1）The percentage of CD8^＋ CD28^＋ lymphocytes of ASC （10.64 ＋ 5.09%） was significantly lower than that of chronic hepatitis B and controls. There was no significant difference in CD45RO and CIM5RA expression at the CD8^＋ lymphocytes of all groups. （2）The CD8 ^＋/CD45RA^＋/CD28^＋ in chronic hepatitis B （10.99±7.33%） group was significantly higher than that of control, while the CD8^＋/CD45RA^＋/CD28^＋ in chronic hepatitis B and ASC group were lower than that of control. （3）The CD8^＋/CD45RO^＋/CD28^＋ in HBeAg negative group was higher than that of HBeAg positive group. But there was no significant difference in CD8^＋ / CD45RA^＋/CD28^＋ .Conclusion The lower expression of the costimulation CD28 in the CD8^＋ lymphocytes are relative to the HBV asymptomatic carriers. The elevation of CD8^＋/CD28^＋ CD45RO^＋ subtype was probably related to ＇the progress of chronic hepatitis B and the serum HBeAg conversion.