局部注射多柔比星脂质体抑制乳腺癌模型腋窝淋巴结转移癌细胞增殖

Inhibition on proliferation of metastatic cells in axillary lymph node with liposomal adriamycin locally injected into a breast cancer model

目的观察局部注射多柔比星脂质体(LADR)对VX2兔乳腺移植癌模型腋窝淋巴结转移癌细胞增殖的抑制作用。方法30只荷乳腺癌兔平均分为3组,1组不给予特殊治疗;2组肿瘤周围皮下注射LADR;3组静脉注射多柔比星(ADM)水溶剂。3次治疗后切除乳腺肿瘤及腋窝淋巴结,比较治疗前后淋巴结体积变化,RT-PCR检测肿瘤及淋巴结增殖细胞核抗原(PCNA)mRNA表达。结果各组腋窝淋巴结平均生长率分别为3.70%、1.55%和2.89%,第3组显著低于第1组(P=0.004),第2组又显著低于第3组(P=0.002)。1、2、3组乳腺肿瘤PCNA相对表达值分别为0.486、0.513和0.396,以第3组为最低,与第1、2组相比差异有统计学意义,P=0.023,P=0.005。腋窝淋巴结PCNA相对表达值分别为0.541、0.329、0.450,第3组显著低于第1组(P=0.021),第2组又显著低于第3组(P=0.004)。结论局部注射LADR是治疗乳腺癌淋巴转移的有效方法,但对原发灶无明显疗效。

OBJECTIVE： To assess the inhibitory effect on the proliferation of lymph node metastases with local injection of liposomal adriamycin （LADR） into rabbits bearing VX2 tumors in the mammary gland. METHODS： Thirty breast-tumor-bearing rabbits were divided into three groups, with 10 rabbits in each. Group 1 received a sham treatment. Group 2 received subcutaneous injection of LADR adjacent to tumor. Group 3 received intravenous injection of free adriamycin （FADR）. The breast tumor and axillary lymph node were resected after the treatment was repeated three times. Node sizes before and after treatment were measured. Proliferating cell nuclear antigen （PCNA） mRNA expressions in breast tumors and lymph nodes were determined by RT-PCR. RESULTS： The mean growth ratios of axillary lymph nodes in groups 1, 2 and 3 were 3.70%, 1.55%, and 2.89%, respectively, with significant difference between groups 3 and 1 （P=0. 004）, and further difference be tween groups 2 and 3 （P=0. 002）. The relative values of PCNA mRNA expressions in breast tumors in groups 1, 2, and 3 were 0.486, 0. 513, and 0. 396, respectively. Group 3 expressed a sufficiently lower level of PCNA mRNA compared to groups 1 （P=0. 023） and 2 （P=0. 005）. The PCNA mRNA expressions in axillary lymph nodes in groups 1, 2, and 3 were 0. 541, 0. 329, and 0. 450, respectively. Compared with group 1, group 3 showed a markedly decreased level of PCNA expression （P = 0. 021）. The lowest expression level was found in group 2, with a further significant difference from group 3 （P=0. 004）. CONCLUSION： Local injection of LADR appears to be an effective method in the treatment of lymphatic metastases from breast cancer, regardless of its little influence on the primary tumor.