肺癌患者Th1和Th2类细胞因子的基因检测及其与化疗的关系

Gene expressions of Th1 and Th2 cytokines in peripheral blood of patients with lung carcinoma and relationship between their expression and chemotherapy

目的观察肺癌患者Th1和Th2类细胞因子的基因表达与化疗的关系。方法采用逆转录聚合酶链反应(RT-PCR)技术,检测102例肺癌患者在化疗前、化疗后外周血单个核细胞(PBMC)Th1和Th2类细胞因子mRNA表达水平。结果肺癌组IL-4、IL-6和IL-10mRNA阳性表达较正常对照及良性疾病组显著增高(P≤0.001。肺癌组IFN-γ和IL-2mRNA阳性表达较正常对照及良性疾病组显著降低(P<0.05)。化疗有效组化疗后IFN-γ和IL-2mRNA阳性表达分别为42/67和43/67,较化疗前显著增高(P≤0.001;IL-4、IL-6和IL-10mRNA阳性表达分别为30/67、26/67和24/67较化疗前显著降低(P≤0.01。化疗无效组化疗前后Th1和Th2类细胞因子mRNA表达差异无统计学意义(P>0.05)。结论肺癌患者Th1类细胞因子表达受抑,Th2类因子呈强势表达;化疗可使肺癌患者Th2类细胞因子的强势表达向Th1类逆转。

OBJECTIVE： To investigate the gene expressions of Thl and Th2 cytokines in peripheral blood mononuclear cells （PBMCs） from patients with lung carcinoma, and evaluate the effect of chemotherapy on them. METHODS： The gene expressions of Thl and Th2 cytokines before and after chemotherapy in 102 patients with lung carcinoma was detected RT-PCR. RESULTS： The positive expression rates of IL-4, IL-6 and IL-10 in lung carcinoma were significantly higher than those of benign pulmonary diseases and the normal controls （P≤0. 001）, The positive expression rates of IFN-γ and IL- 2 mRNA in lung carcinoma were significantly lowerer than those of benign pulmonary diseases and the normal controls （P≤0.05）, After chemotherapy,the positive rate of IFN-γ and IL-2 mRNA in the group seneitive to chemotherapy of lung carcinoma was a significant enhancement than those of before chemotherapy （P≤0. 001）, The positive rate of IL-4, IL-6 and IL-10 mRNA was a significant descend than those of before chemotherapy （P〈0.01）. There was no difference in the expression of Thl and Th2mRNA in the group no sensitive to chemotherapy （P〉0.05）. CONCLUSION： The expression of Th1 cytokines was inhibited with a concomitant predominant state of Th2 cytokines in PBMCs from patients with lung carcinoma. Chemotherapy can convert the imbalance state of Th1 and Th2 cytokines and make it incline to the normal state.