PTEN与FHIT在骨肉瘤组织中的表达

Expressions of PTEN and FHIT in osteosarcoma

目的探讨骨肉瘤组织中抑癌基因PTEN、FHIT的表达情况。方法应用免疫组织化学SP法测定PTEN、FHIT在45例骨肉瘤、20例骨软骨瘤和10例正常骨组织中的表达情况,观察骨肉瘤中PTEN、FHIT的表达与临床预后之间的关系。结果45例骨肉瘤、20例骨软骨瘤、10例正常骨组织中PTEN阳性率分别为24.4%、95.0%和100%;FHIT阳性率分别为15.6%,95.0%和100%。骨肉瘤与骨软骨瘤及正常骨相比均有差异有统计学意义,P<0.001,骨肉瘤中PTEN、FHIT表达下调者易复发转移、生存时间短,P<0.001。结论PTEN、FHIT在骨肉瘤组织中表达下调,可作为评估骨肉瘤的发生和预后的指标。

OBJECTIVE： To investigate the expressions of PTEN and FHIT in osteosarcoma. METHODS： The immunohistochemical staining method was used to evaluate the expressions of PTEN and FHIT in 45 cases of osteosarcoma, 20 cases of osteochndroma and 10 cases of normal osseous tissues. RESULTS： The positive rate of PTEN were 95.0% in osteochndroma, 24.4% in osteosarcoma and 100% in normal osseous tissues, P〈0. 001. The positive rates of FHIT were 95.0% in osteochndroma, 15.6% in osteosarcoma and 100% in normal osseous tissues. The positive rates of FHIT and PTEN were significantly lower in osteosarcoma than those in osteochndroma and normal osseous tissues, P〈0. 001. The downregulation of PTEN and FHIT expressions was correlated with metastasis and poor prognosis. CONCLUSION： Downregulation of PTEN and VHIT expressions can be used to evaluate the pathogenesis and prognosis in osteosarcoma.