高效液相色谱法测定甲磺酸帕珠沙星注射液含量

Determination of Pazufloxacin Mesilate and Sodium Chloride Injection by HPLC

目的建立甲磺酸帕珠沙星注射液中甲磺酸帕珠沙星的含量测定方法。方法高效液相色谱法、C18柱、以乙腈-[10％甲磺酸溶液。1．0mol·L^-1磷酸氢二钾溶液-水（10：7：153）（用三乙胺调节pH值至3．0）]（30：170）为流动相、检测波长为244nm。结果甲磺酸帕珠沙星在20—80μg·ml^-1浓度范围内呈良好的线性关系（r=0．9999），平均回收率为100．26％，RSD为0．52％。结论本法准确可靠、方便可行，可作为甲磺酸帕珠沙星注射液中甲磺酸帕珠沙星的含量测定方法。

Aim To establish a method of determining of Pazufloxacin Mesilate in Pazufloxacin Mesilate Injection. Methods An HPLC method with chromatographic conditions involved C18 column and the mobile phase consisting of CH3CN-（ 10% methanesulfonic acid solution-1.0mol ·1^-1 di-potassium hydrogen phosphate trihydrate solution-H2O） （10： 7： 153）（ adjusted with tyiethylamine to a pH of 3.0）（30： 170） . The detection wavelength was 244nm. Results Pazufloxacin Mesilate had a good linearity（ r =0.999 9） within the range of 20 - 80μg·ml^-1. The average recovery was 100.26% and the RSD was 0.52% o Conclusion This method is accurate and easy to use for determination of the content of Pazufloxacin Mesilate Injection.