中亚以东南绵羊群体钾座位遗传分化的研究

Study on genetic differentiation in Ke locus of sheep populations in east and south of central Asia

利用中心产区典型群简单随机抽样法,以湖羊、同羊、滩羊、小尾寒羊、洼地羊为研究对象,引用我国周边国家、地区绵羊品种资料为分析背景,采用Na/K/Cl离子分析仪对红细胞中钾的含量进行测定。结果表明:1)各群体钾座位中立性检测的观察值除Bhyanglung绵羊外,其余均在L95和U95之间,表明钾座位作为中立性座位,基本未受到选择等因素的影响;2)血钾基因频率在地理分布上存在一定规律,基本符合将中亚以东南固有绵羊系统划分为“蒙古羊”、“藏羊”和“南亚-东南亚羊”三大集团;3)中亚以东南16个绵羊群体的钾座位基因分化系数为0.185 9。这说明上述16个绵羊群体钾座位81.41%的变异是由群体内的遗传多态现象引起的,只有18.59%的变异来自于群体间的差异。

The Chinese native sheep populations including Tan sheep, small-tailed Hart sheep, Hu sheep, Tong sheep and Wadi sheep randomly sampled in typical colonies of central area were studied with other 10 Asian native sheep populations around China by foreign scholars as the analyzed background. The blood potassium phenotype was examined by a Na-K-Cl analyzer. The results showed that： 1） the values of Ewens-Watterson test for neutrality of Ke locus, except for Bhy, located between L95 and U95, which indicated that as a neutral locus Ke was not affected by the selection; 2） some trends on blood potassium gene frequencies in geography distribution were founded that accorded with the classification of east and south of central Asian sheep systems, ＂Mongolian sheep＂, ＂Tibetan group＂ and ＂South Asia-Southeast Asia group＂; 3） Gst of Ke locus of 16 sheep populations in east and south of central Asian was 0. 185 9, which indicated that 8l. 41% of variations of Ke locus in the 16 populations were caused by genetic polymorphism within populations and only 18.59% due to the difference among populations.