河北杨和新疆杨离体叶片诱导不定芽研究

Studies on adventitious buds induction in vitro leaf of Populus hopeiensis Hu et Chow and Populus alba L.var.pyramidali

对河北杨和新疆杨离体叶片诱导不定芽的方法进行了研究。结果表明，适合河北杨和新疆杨离体叶片不定芽诱导的最适培养基为：1／2MS＋0．25mg／L 6-BA＋0．01mg／LTDZ＋0．25mg／L IAA;在此培养基中，叶片分化率河北杨可达70％，新疆杨可达52％；平均分化芽数河北杨为6．84个，新疆杨为5．32个。6-BA单独使用对叶片不定芽诱导没有效果，NAA作用不明显。叶片刻伤方式对两种杨树叶片诱导不定芽影响显著，沿中脉横切效果优于去除叶边缘，诱导的不定芽数前者比后者约多3个。光照显著影响叶片不定芽产生的时间，不定芽在3～5d暗培养后转至16h／d光照下培养比全程16h／d光培养发生早15d左右；光照对分化率和平均分化芽数无显著影响。

Studies on adventious buds induction in vitro leaf of Populus hopeiensis Hu et Chow and P. alba L. var. pyramidali were carried out. The systematic experiment showed that 1/2 MS^＋ 0. 25 mg/L 6-BA ＋ 0. 01 mg/L TDZ＋0. 25 mg/L IAA was the optimal system to induce adventitious buds of Populus hopeiensis Hu et Chow and P. alba L. var. pyramidalis in vitro leaves. In this medium, 70% leaves of P. hopeiensis Hu et Chow and 50% leaves of P. alba L. var. pyramidalis produced adventitious buds and the average numbers of each were 6.48 and 5.32 respectivelly. The study also showed that only 6-BA had no influence on the induction of adventitious buds. NAA had a little effect on the formation of adventitious buds. The means of cutting leaves had remarkable effect on the of formation adventitious buds,and the numbers of adventitious buds describing the leaves＇ middle ventions were three times more than disposing the leaves＇ edge. Illumination would influence the time of leaves to induce adventitious buds but had no effect on the differentiation rate and average numbers of the adventitious buds. The adventitions buds were induced 15 days earlier with 3-5 days＇ dark culture and then 16 hours＇ illumination per day than with 16 hours＇ illumination per day.