HPLC测定小儿退热中空栓中绿原酸含量

The Method of Determining Caffeoylquinic Acid in the Xiao er Hollow Type Suppository by HPLC

目的建立小儿退热中空栓含量测定方法。方法色谱柱：YMC—Pack ODS—C18柱（250×4．6mml-D-S-5μm，12nm No．042577874（W）；流动相：乙腈～0．4％磷酸（13：87）；流速为1ml·min^-1；检测波长；327nm；柱温30℃。结果绿原酸在0．0832-2．08μg范围内呈良好的线性关系，精密度RSD1．07％，重复性RSD为1．39％，稳定性RSD为0．79％．回收率RSD为0．8％，含量测定RSD为2．29％。结论本法简便、可靠、重现性好、可用于该制剂的质量控制。

OBJECTIVE To set up assasylng method of Xiao＇er tui re zhong kong suan. METHODS Chromatographic column ： YMC-Pack ODS-C18柱（250 × 4.6mml. D. S-5μm, 12nm No. 042577874 （W） ; mobile phase of eaffeoylquinic acid： 0.4%H3PO4 （13 ： 87）; flow rate： 1ml· min^-1 detective wavelength： 327nm; columntemperature30℃. RESULTS Caffeotannic acid has good linear relationship between0. 0832 -2.08μg, RSD % of precision： 1.07 %, RSD% of reproducibility ： 1.39 %, RSD% of stability ： 0. 79 % RSD % of recovery ： 0.8%,assaying RSD% 2.29%. CONCLUSION The method is convenient,reliability,good reproducibility,can be used in the quality control of the preparation.