摘要
目的：建立RP—HPLC法测定北柴胡地上部分山柰酚含量的色谱方法。方法：采用RP—HPLC，C18拄（150mm×4．6mm，5μm），流动相甲醇-（0．5％磷酸）水（体积比为48：52），检测波长360nm，柱温34℃。结果：山亲酚质量浓度在0～0．048g／L内与峰面积呈良好的线性关系，r=0．9995（n=6）；平均回收率为100．7％，RSD=1.8％（n=6）。结论：测定方法准确，可靠，为北柴胡地上部分的质量评价提供了可靠的依据。
To establish a HPLC method for the determination of kaempferol in the aerial part of Bupleurum chinense DC. Methods：A C18 column（ 150 mm × 4.6 mm, 5μm）with a solvent system consisting of methanol - （0.5% phosphoric acid）water （ V： V = 48：52） was used, and the detecting wavelength was set at 360 nm. The column temperature was 34 ℃. Result ： The calibration curve was linear over the range of 0 - 0. 048 g/L with the correlation 0.999 5（ n = 6）.The average recoveries of kaempferol was 100.7% with RSD being of 1.8 % （ n = 6）. Conclusion： The method is sensitive, accurate, rapid, and is suitable for the quality control of the aerial part of Bupleurum chinense DC.
出处
《中国野生植物资源》
2006年第6期61-62,共2页
Chinese Wild Plant Resources
