促肝细胞生长素对肾缺血-再灌注损伤大鼠NO及肾脏病理学的影响

Effects of Hepatocyte Growth-Promoting Factor on NO and Pathological Lesions in Rats with Renal Ischemia Reperfusion Injury

目的观察促肝细胞生长素(Hepatocyte Growth-promoting Factor,pHGF)对大鼠肾缺血-再灌注损伤后血清NO、Scr及肾脏组织病理学的影响。方法雄性Sprague-Dawley大鼠32只,随机分为假手术对照组(Ⅰ组)、缺血再灌注组(Ⅱ组)、缺血再灌注前pHGF干预组(Ⅲ组)和缺血再灌注后pHGF干预组(Ⅳ组)。用无损伤动脉夹钳夹大鼠双侧肾蒂45min制成肾缺血-再灌注损伤(renal ischemia reperfusioninjury,RIRI)模型。假手术组只暴露双肾,不钳夹肾蒂。Ⅲ组和Ⅳ组分别在术前和术后腹腔注射pHGF50mg/kg。检测术后12h血清NO和Scr的浓度及病理切片观察肾脏组织病理学改变。结果Ⅰ组血清NO、Scr水平均为最低,Ⅱ组各值均显著升高,两组比较各项均有显著差异。Ⅲ组和Ⅳ组各项数值较Ⅱ组均有一定程度的降低,两组分别与Ⅱ组比较均有显著差异。但Ⅲ组和Ⅳ组之间比较无显著差异;缺血再灌注12h肾脏病理改变最严重,肾小管损伤程度评分最高,pHGF干预后,肾脏形态学改变明显减轻,肾小管损伤程度评分降低;血清NO水平与肾功能及肾脏损害呈正相关。结论pHGF能抑制肾缺血-再灌注损伤大鼠血清NO水平,对IARF在肾脏结构及功能上既有保护作用又有治疗作用。

Objective To investigate Nitric Oxide （NO） expression and the effects of Hepatoeyte Growth -promoting Factor （pHGF） in renal ischemia reperfusion injury in rats. Methods 32 male Sprague- Dawley rats were random divided into four groups （n= 8） ：Sham - operated control group （ Ⅰ group） ; Ischemia reperfusion group （ Ⅱ group） ; Injecting pHGF before operation group （ Ⅲ group） ; Injecting pHGF after operation group （ivgroup） .The animal model of IRI was induced by clamping bilateral renal pedical for 45 min.Bilateral renal pedical of rats inⅠ group were not clamped, pHGF were injected into abdominal cavity of rats in Ⅲ and Ⅳ groups before and after operation respectively.To assay blood NO, serum creatine （Set） and to observe renal tubular epithelial cells injury degree by pathology at IR 12h. Results The level of blood NO, Scr and renal tubular epithelial cells injury degree score in Ⅱ group was significamly higher than those of I group （P 〈 0.01 ） .The level of blood NO, Scr and renal tubular epithelial cells injury degree score in Ⅲ and Ⅳ groups was decreased significantly in comparison with those in Ⅱ group, and the pathological lesions in kidney were attenuated, but the results were not significant statistically between Ⅲ and Ⅳ groups （P 〉0.05）. The level of NO was positive related with Scr and renal tubular epithelial cells injury degree score respectively （r = 0. 846, P 〈 0.01;r = 0. 797, P 〈 0.01 ）. The level of Scr was also positive related with renal tubular epithelial cells injury degree score （r = 0.938, P 〈 0.01 ）. Conclusion These results suggest that NO may play an important role in renal ischemia reperfusion injury.pHGF can significantly attenuate the extent of RIRI through lowering the contents of NO. pHGF not only protect but treat the rats with RIRI.