超临界CO_2合成鱼油质构脂质中酰基转移的研究

Acyl Migration of Fish Oil Structured Lipids Synthesized in Supercritical Carbon Dioxide

超临界CO2酶法合成鱼油质构脂质的酯化反应中,反应参数的控制影响酰基转移。当酶的水含量为30%,酶用量为底物总量的10%,辛酸乙酯和鱼油摩尔比为12.5∶1,反应温度为45℃,反应压力为12.5MPa,反应时间为11h时,采用格里雅降解法测定鱼油质构脂质中辛酸的酰基转移量为1.5%。

This work deals with the enzymatic interesterification of fish oil structured lipids which is synthesized in supcrcritical carbon dioxide. The influence of the reaction factors on the acyl migration was studied. The result shows that with lipase water content 30% , immobilized lipasc 10% of substrate, molar ratio of ethyl caprylate to fish oil 12.5：1, reaction temperature 45℃, 12.5 MPa and reaction time 11h, the acyl migration of caprylic acid measured by Grignard degradation is 1. 5%.