He-Ne激光照射对脾巨噬细胞吞噬功能影响的图像分析研究

IMAGE ANALYSIS OF He-Ne LASER IRRADITION ON THE PHAGOCYTOSIS OF SPLENIC MACROPHAGES IN MICE

目的:研究He-Ne激光对小鼠免疫功能的影响。方法:用剂量63.7 J/cm2的He-Ne激光照射小鼠脾区,照射时间为5、10d,每天照射5m in,采用锥虫蓝活体注射显示巨噬细胞吞噬力试验方法,观察脾巨噬细胞吞噬功能,并用图像分析法对吞噬颗粒进行定量分析,所有数据输入微机作F检验。结果:照射组巨噬细胞吞噬锥虫蓝的颗粒数目、颗粒面积、颗粒面积与细胞面积之比,颗粒的积分光密度四项指标均较对照组明显增高,差异有非常显著性(P<0.01)。结论:适当剂量的He-Ne激光照射小鼠脾区,可激活脾内巨噬细胞吞噬功能,提高机体的免疫力。

Objective：To investigate the effects of He - Ne laser on immune function of mice.Methods ： The area over spleen of the mice was irradiated with the He - Ne laser at the dosage of 63.7 J/cm^2 for 5,10 days respectively, lasting 5 minutes each day . Then observing the phagocytosis of the splenic macrophages after injecting live mice with trypan blue, and making quantitative analysis of macrophages phagocytosis by image analysis system. Results： The four indices： number of trypan granule, area of granule, ratio of granule area to cell area and granule integral optical density at irradiated groups were higher than that of control group （P 〈 0. 01 ） . Comparison between different irradiated groups also showed significant difference （ P 〈 0. 01 ）. Conclusion ： He - Ne laser at appropriate dosages may activate macrophages , and enhance the immunity of organism .