MRSA、MSSA对4类抗菌药物耐药基因的研究

Resistance Genes of Antibacterial Agents in MRSA and MSSA

目的探讨耐甲氧西林金黄色葡萄球菌(MRSA)和甲氧西林敏感金黄色葡萄球菌(MSSA)耐药基因存在状况。方法聚合物酶链反应(PCR)法检测mecA、ermA/B/C、aac(6′)/aph(2″)、aph(3′)-Ⅲ、ant(4′,4″)t、etM等耐药基因。结果MRSA检出了mecA、ermA/B/C、aac(6′)/aph(2″)、aph(3′)-Ⅲ、tetM等5种耐药基因。结论MRSA是多重耐药细菌。

OBJECTIVE To investigate the condition of drug-resistant genes in MRSA and MSSA. METHODS The drug-resistant genes mecA, ermA/B/C, aac（6′ ）/aph （2″）, aph （3′ ）-Ⅲ, ant （ 4′, 4″） and tetM of MRSA and MSSA were detected by polymerase chain reaction（PCR）. RESULTS The 5 kinds of drug-reslstant genes, such as mecA, ermA/B/C,aac（6′）/aph（2″）,aph（3′）- Ⅲ and tetM were positive in MRSA. CONCLUSIONS MRSA is a multi-resistant pathogen.