摘要
目的应用随机扩增多态性DNA(RAPD)技术,对耐亚胺培南铜绿假单胞菌进行基因多态性研究。方法对医院临床分离的8株耐亚胺培南铜绿假单胞菌进行随机引物聚合酶链反应(PCR)扩增,扩增产物进行电泳和聚类分析。结果耐亚胺培南铜绿假单胞菌耐药表型相同,但基因型相同,绝大多数基因型不同。结论通过RAPD分析了解耐亚胺培南铜绿假单胞菌基因型的特性,明确耐亚胺培南铜绿假单胞菌耐药的表型与基因型的关系,为该菌的感染控制提供分子流行病学依据。
OBJECTIVE To investigate the genetic polymorphism of imipenem-resistant Pseudomonas aeruginosa by randomly amplified polymorphic DNA(RAPD). METHODS The genes of imipenem-resistant P. aeruginosa were amplified by RAPD assay in 8 clinical isolates and PCR products were analyzed by agarose gel electrophoresis and cluster analysis. RESULTS The phenotypes of drug resistance were the same, but most of the genotypes were different, even though some of them could be equal. CONCLUSIONS RAPD assay reveals the special genotypes of imipenem-resistant P. aeruginosa and makes the relation between the phenotype of drug resistance with genotype of drug resistance definite, which provides molecular epidemiological evidence for strategies to control resistant imipenem P. aeruginosa infections.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2006年第12期1335-1336,共2页
Chinese Journal of Nosocomiology