医院感染铜绿假单胞菌菌株亲缘性分析

Relative Analysis of Pseudomonas aeruginosa Strain in Hospital Infection

目的了解医院感染铜绿假单胞菌(PAE)菌株的亲缘性。方法聚合酶链反应(PCR)法对多重耐药的铜绿假单胞菌进行β-内酰胺酶基因、氨基糖苷类修饰酶(AMEs)基因、耐消毒剂基因(qacE△1-sul1)的检测,并以耐药基因为分子标志作聚类分析。结果TEM、CARB、VIM的阳性率分别为24.32%、43.24%、5.40%,而SHV、CTX-M、OXA-10 group、PER、GES、VEB、DHA、IMP基因均阴性;oprD2缺失率为86.49%;aac(6′)-Ⅰ、aac(6′)-Ⅱ、ant(3″)-Ⅰ和ant(2″)-Ⅰ阳性率分别为8.11%、43.24%、18.91%、40.54%、qacE△1基因为51.35%,存在克隆传播现象。结论医院感染铜绿假单胞菌可导致克隆传播医院感染,并存在暴发性流行。

OBJECTIVE To investigate the relationship of Pseudomonas aeruginosa strain in hospital infection. METHODS β-Lactamase gene, aminoglycoside modification enzymes （AMEs） gene, and resistant antiseptic （qacE△1-sul1）gene were detected to mutidrug resistant P. aeruginosa with polymerase chain reaction （PCR） and we conducted cluster analysis of the molecule marked drug resistance gene. RESULTS PCR showed the positive ratios of TEM,CARB and VIM genes were 24.32 %, 43. 24%, and 5.4 %, respectively, While SHV, CTX-M,OXA-10 group,PER,GES, VEB, DHA, and IMP genes were all negative, the deleted ratio of oprD2 gene was 86.49 % ; the positive ratios of aac（6′）-Ⅰ , aac（6′）-Ⅱ , ant（3″）-Ⅰ and ant（2″）- Ⅰ genes were 8. 11%, 43.24%, 18. 91%, and 40.54%, respectively and the positive ratio of qacE△1 gene was 51.35%, There were clone transmitted phenomenon. CONCLUSIONS Hospital P. aeruginosa infection can induce clone transmitted hospital infection and it has fulminant prevalence.