3种含氯消毒剂对手术器械预消毒效果的比较研究

Disinfection Effect of Three Kinds of Chlorine-containing Disinfectants on Surgical Instruments

目的对3种常用含氯消毒剂用于手术器械预消毒,进行效果比较研究。方法分别用3种消毒剂对污染了乙型肝炎病毒(HBV)的手术器械进行预消毒,运用聚合酶链反应(PCR)和酶联免疫吸附(ELISA)两种分子生物技术,分别检测HBV-DNA和乙型肝炎表面抗原(HBsAg),观察HBV灭活情况,比较3种消毒剂的消毒效果。结果用于手术器械预消毒的3种消毒剂均有一定效果,消佳净20/500浓度组效果相对最好,但HBV阳性率仍然较高。结论含氯消毒剂用于术后手术器械预消毒,可减少器械准备人员医源性感染的风险,但仍须加强个人保护。

OBJECTIVE To study the disinfection effect of three kinds of chlorine-containing disinfectants to surgical instruments. METHODS HBV on surgical instruments disinfected respectively by three kinds of disinfectants were tested with PCR and ELISA. RESULTS Although HBV negative proportions were not too high, three kinds of chlorine-containing disinfectants had disinfection effect on surgical instrument. The HBV negative proportion of XIAOJIAJIN 20/500 group was 71.4% （P〈0.05）, and the highest among the groups. CONCLUSIONS The predisinfection with chlorine-containing disinfectants could reduce the infection risk among medical staff for disinfecting and preparing the surgical instruments, hut they should still pay attention to protect themselves.