杀虫剂的选择作用对小菜蛾表达蛋白质的影响

Protein Changes in Response to Insecticides Selection Pressure in Diamondback Moth

运用蛋白质双向电泳分析技术，通过对成虫蛋白质的提取，分离出水溶性的蛋白质组分，对比研究了溴氰菊酯抗性品系、杀螟丹抗性品系、杀虫双抗性品系和敏感品系小菜蛾成虫的蛋白质在表达上的差异．通过对电泳图谱分析，在澳氰菊酯的抗性和敏感品系中抉得了8个特异的蛋白质斑点，pH5．0～8．1，相对分子质量为46000～23000；在抗杀螟丹品系中得到了8个特异斑点，pH4．6～81，相对分子质量为92000～18000；在杀虫双的执性品系和敏感品系中产生了6个特异斑点，pH5．5～7．1，相对分子质量为31000—28000．虽然抗性品系是敏感品系通过多年的室内逐代的药剂选掸获得的，但是在杀虫刺选择压力作用下所引起的表达蛋白质的差异是否与浪氰菊酯、系螟丹和杀虫双的抗性有关还需进一步证实．

The diamondback moth, Plutella xylostella （L.）, has developed resistance to various insecticides. Changes induced in moth proteins of one susceptible strain and three strains selected for resistance to deltamethrin, cartap and dimehypo, respectively, were compared with protein＇ s two-dimensional gel electrophoresis. After the analysis of the spectrum of gel electrophoresis, eight proteins, pH 5.0 - 8.1 and molecular weight 46 000 - 23 000, showed different expressions in deltamethrin resistant strain and its susceptible strains ; Eight proteins, pH 4.6 - 8.1 and molecular weight 92 000 - 18 000, were expressed specially in cartap resistant strain; and Six proteins, pH 5.5 - 7.1 and molecular weight 31 000 - 28 000, were expressed differently in dimehypo resistant strain and its susceptible strains, Though all resistant strains were selected by the action of insecticides on the basis of susceptible strain, whether these different expressed proteins are related to the resistance to dehamethrin, eartap and dimehypo should be studied further.