光叶楮组织培养快速繁殖技术的研究

Research on in vitro rapid micropropagation of Bronssonetia papyrifera (L.) Vent.

对光叶楮组织培养中的外植体灭菌、苗玻璃化预防、增殖培养、根原基诱导及室外移栽进行的试验结果表明：以优良母株的具腋芽茎段为外植体，经过0．1％的HgCl2灭菌后，外植体成活率为53．6％；培养基中添加4．0％的蔗糖及增加培养容器的透气性能能较好地预防玻璃苗的发生；在MS培养基中附加BA，NAA等植物生长调节剂可有效促进试管苗的增殖和生长，其中以1．5mg／LBA＋0．15mg／LNAA的组合最为适宜；适宜的根原基诱导培养基为1／2MS＋0．5mg／LIBA＋0．3mg／LNAA＋100mg／LLH＋2％蔗糖；具根原基试管苗移栽于混合基质（蛭石：珍珠岩：泥炭=6：3：1，体积比）中，温度控制在20—30℃，相对湿度为85％～90％，保湿15～20d，其间适当遮荫，30d后试管苗成活率达90％。

The experiments in Bronssonetia papyrifera（ L. ）Vent. were committed concerning explant sterilization, shoot vitrification control, multiplication cultrue, root promordia induction and transplant. Results showed that with the nodal segments from the elite parent trees of Bronssonetia Papyrifera （ L. ） Vent. as explants, their survival could get 53.6% after 0. 1% HgCl2 sterilization; addition of 4.0% sucrose （S） to media and container air-permeability could effectively control shoot vitrification; MS supplemented with BA and NAA could improve culture multiplication and growth, with the media containing 1.5 mg/L BA ＋ 0. 1 5mg/L NAA optimal;the optimal root promordia induction took place in 1/2MS media containing 0. 5 mg/L IBA ＋0. 3 mg/L IBA ＋ 100 mg/L LH ＋2% S; transplant of the shoots with root promordia into the substrate mixed with 6： 3：1 （ volume ratio） vermiculite ,perlite and peat in 20 - 30℃, under relative humidity 85% - 90% for 15-20 d, preferrable shading occasionally, could get the transplant survival up to 90% after 30 d.