端粒酶在结肠良、恶性病变中的表达及其意义的研究

Telomerase＇ s expression in benign and malinant lesions of colorectum and their clinical significance

目的 研究端粒酶在正常结肠黏膜、结肠良、恶性病变中的表达及其临床意义。方法 收集27例结肠癌，9例结肠良性病变和13例正常肠黏膜的手术标本，用半定量端粒重复扩增（TRAP）-银染法测端粒酶活性，进行统计学处理。结果 结肠正常黏膜、良、恶性病变的端粒酶相对值的组间两两比较均有显著性差异（P〈0．05）。将结肠癌病人分别按病理类型、Duke分期病变体积、部位、病人病程、年龄、性别进行分组，各组间两两比较均没有显著性差异（P〉0．05）。结论 端粒酶是结肠正常黏膜、良性病变、结肠癌相鉴别的较好辅助诊断指标，与结肠癌的一些临床资料无关。

AIM To study the expression of telomerase innormal mucosee, benign and malignant lesions of colorectum and their clinical significance. METHODS 49 specimens （27 cases of carcinomas,9 cases of benign lesions and 13 cases of normal mucosae of colorectum） were collected. Telomerase activity was examined using the new silver staining semi - quantitative telomere repeat amplification protocal（TRAP） method. The data was taken to statistical analyses according to the variant type. RESULTS There were significant difference between the groups of normal mucosa,benign lesion and carcinoma of colorectal telomerase relative indexes. There were no statistical significances between telomerase activity and clinical factors for colorectal carcinoma（ P 〉 0.05）. CONCLUSIONS Telomerase is a good diagnosis narker of distinguishing the normal mucosa, benign and malignant lesions of colorecturn. But telomerase activity have no relations with certain clinical factors for colorectal carcinoma.