摘要
探讨了培养基成分、培养时间、高渗缓冲液成分和ｐＨ、溶菌酶浓度以及酶解温度和时间对苏云金杆菌ＳＤ—５菌株原生质体形成和再生的影响；结果表明，ＳＤ—５菌株原生质体形成和再生的最佳条件组合为：ＰＢＧ培养基、３０℃培养１４ｈ再活化５ｈ，以ＳＭＭｐＨ６．５作酶解缓冲液，溶菌酶浓度０．６ｍｇ／ｍｌ，３４℃处理６０ｍｉｎ．
The effects of different factors,such as medium compositon,culture period,hypertonic buffer solution,pH,lysozyme dose,lemperature and enzymolysis time,on protoplast formation and regeneration of Bacillus thuringiensis strain SD 5 were studied.The best protoplast formation and regeneration condition was PBG medium,cultivated for 14 hours and activated for another 5 hours,SMM hypertonic buffer,pH6.5,lysozyme dose 0.6 mg/ml,34℃ for 60 minutes.
出处
《山东师范大学学报（自然科学版）》
CAS
1996年第3期77-81,共5页
Journal of Shandong Normal University(Natural Science)
基金
山东省科委资助
关键词
苏云金杆菌
原生质体
形成
再生
培养基
Bacillus thurinqiensis
SD 5
protoplast
formation and regeneration
