摘要
探讨了培养基成分、培养时间、高渗缓冲液成分和pH、溶菌酶浓度以及酶解温度和时间对苏云金杆菌SD—5菌株原生质体形成和再生的影响;结果表明,SD—5菌株原生质体形成和再生的最佳条件组合为:PBG培养基、30℃培养14h再活化5h,以SMMpH6.5作酶解缓冲液,溶菌酶浓度0.6mg/ml,34℃处理60min.
The effects of different factors,such as medium compositon,culture period,hypertonic buffer solution,pH,lysozyme dose,lemperature and enzymolysis time,on protoplast formation and regeneration of Bacillus thuringiensis strain SD 5 were studied.The best protoplast formation and regeneration condition was PBG medium,cultivated for 14 hours and activated for another 5 hours,SMM hypertonic buffer,pH6.5,lysozyme dose 0.6 mg/ml,34℃ for 60 minutes.
出处
《山东师范大学学报(自然科学版)》
CAS
1996年第3期77-81,共5页
Journal of Shandong Normal University(Natural Science)
基金
山东省科委资助
关键词
苏云金杆菌
原生质体
形成
再生
培养基
Bacillus thurinqiensis
SD 5
protoplast
formation and regeneration