菊叶薯蓣茎段组织培养研究

Studies on tissue culture of stem segment of Dicorea composita

为解决菊叶薯蓣种苗的繁育问题,用不同种类、浓度的植物生长调节剂对菊叶薯蓣幼嫩茎段进行离体培养试验,结果表明,启动培养用M S+6-BA 0.2m g/L+KT 1.0m g/L+NAA 0.01m g/L诱导不定芽萌芽率为100%,效果最理想;继代培养用M S+6-BA 0.8m g/L+NAA 0.2m g/L诱导芽的增殖系数超过3,长势较理想;生根培养用1/2M S+IBA 1.5m g/L+NAA 0.3m g/L诱导生根长苗率达90%以上,对生根培养的效果最佳;采用河沙+塘泥假植菊叶薯蓣组培生根苗成活率高,生产成本降低;表明应用组培快繁技术能为菊叶薯蓣的规模化种植提供充足的优质种苗。

In order to solve the propagation of Dicorea composite, Dicorea corn posite shoot were cultured in vitro with different type and concentration of plant growth regulators. The restdts showed that the best medium for inducing adventitious bud was MS＋ 6-BA0.2mg/L ＋KT1.0mg/L ＋ NAA0. 01mg/L, and the germination rate of adventitious bud was 100%. The coefficient of multiplication of induced bud was over 3.0 on subculture medium MS＋ 6-BA0. 8mg/L ＋NAA0. 2mg/L, and the growth of induced bud was vigor. The medium I/2MS＋ IBA 1.5mg/L＋NAA0.3mg/L was the best for inducing roots and the rootage rate was over 90%. Moreover, the survival rate of rootage plantlets was higher when transplanted in medium of river sand and pound soil. The production cost was low by using this protocol. Through tissue culture and rapid propagation technology, it would supply good plantlets for Dicorea composite production in large scale.