氟尼辛葡甲胺在鸡体内的药物动力学研究

Study on pharmacokinetics of flunixin meglumine after intravenous,intramuscular and oral administration in broilers

36只健康三黄肉鸡,随机分为3组,进行单次静注、肌注和内服2.2 mg.kg-1氟尼辛葡甲胺,用反相高效液相色谱法测定鸡血浆中氟尼辛葡甲胺的浓度,经3P97药物动力学软件处理血浆药物浓度-时间数据。试验结果表明,静注给药拟合适合二室开放模型,主要动力学参数为:t1/2α:(0.56±0.20)h,t1/2β:(5.98±0.02)h,Vd(area):(0.01±0.003)L.kg-1,CL:(0.03±0.01)L.kg-1.h-1,AUC:(683.00±185.79)μg.h-1.mL-1。肌注和内服给药的药时数据均适合一级吸收二室模型,肌注主要动力学参数为:t1/2α:(0.78±0.04)h,t1/2β:(6.76±0.24)h,Vd(area):(0.01±0.003)L.kg-1,CL:(0.005±0.001)L.kg-1.h-1,AUC:(499.40±130.47)μg.h-1.mL-1,F:73.12%;内服的主要动力学参数是:t1/2α:(0.85±0.11)h,t1/2β:(5.15±0.19)h,Vd(area):(0.02±0.004)L.kg-1,CL:(0.01±0.002)L.kg-1.h-1,AUC:(288.51±74.06)μg.h-1.mL-1,F:42.24%。结果显示:氟尼辛葡甲胺在健康鸡体内的主要药物动力学特征为吸收迅速,达峰时间较短,肌注生物利用度较高,内服生物利用度一般。

Pharmacokineties of flunixin meglumine （FM） was investigated in 36 healthy broilers following single intravenous, intramuscular and oral administration of the drug at the dose of 2.2 mg·kg^-1. Blood samples were collected at different intervals after administration and concentrations of FM were determined by HPLC method with a limit of detection of 0.1 μg·mL^-1. The FM concentration-time data were fitted to a two-compartment open model after single intravenous dosing in broilers. The main pharmacokinetic; parameters were as follows： t1/2α： （0.56±0.20） h, t1/2β： （5.98±0.02） h, Vd （area）： （0.01±0.003） L·kg^-1, CL： （0.03±0.01） L·kg^-1·h^-1, AUC： （683.00±185.79） μg·h^-1·mL^-1. The drug concentration-time data were fitted to a two-compartment model with first order absorption after single imtramuseular administration in broilers, The main pharmaeokinectic parameterswereasfollows： t1/2α： （0.78±0.04） h, t1/2β： （6.76±0.24） h, Vd （area）： （0.01±0.003） L·kg^-1, CL： （0.005±0.001） L·kg^-1·h^-1, AUC： （499.40±130.47） μg·h^-1·mL^-1, F： 73.12%, Pharmacokinetics of FM following oral administration was described by a two-compartment open model with first order absorption. The main parameters were as follows： t1/2α： （0.85±0.11） h, t1/2β： （5.15±0.19） h, Vd （area）： （0.02±0.004） L·kg^-1, CL： （0.01±0.002） L·kg^-1·h^-1, AUC： （288.51±74.06） μg·h^-1·mL^-1, F： 42.24%. The results showed that FM was rapidly absorbed, extensively distributed and slowly eliminated in broilers.