摘要
采用改良硫酸铵盐析法从鸡胆汁中分离鸡分泌型免疫球蛋白A（SIgA）．Macro-prep High Q阴离子交换柱层析提纯，经二次过柱后获得的鸡SIgA浓度达22mg／mL。SDS-聚丙烯酰胺凝胶电泳检测表明，重链分子质量为63～64ku，轻链为26～28ku，J链约16ku。用纯化的鸡SIgA免疫兔，所得的抗血清经双向琼脂扩散，证明兔抗鸡SIgA血清效价达1：128。
The improved method of ammonium sulfate was employed to isolate chicken secretary immunoglobulin A （SIgA） from the galls of chicken. Then SIgA was purified twice by Macro-prep High Q ion-exchange chromatography, and 22 mg/mL SIgA had been obtained. Sodium sulphate polyacrylamide gel electrophoresis indicated that the molecular weight of heavy chains,light chains and joining chains were about 63-64 ku, 26-28 ku and 16 ku, respectively. Rabbit antiserum from inoculated rabbits by purified SIgA is proved by double agar diffusion test that the titer of the antiserum reaches to 1 ： 128.
出处
《广西农业生物科学》
CAS
CSCD
2006年第4期290-293,共4页
Journal of Guangxi Agricultural and Biological Science
