阻断C-KIT导致小鼠小肠基本电节律紊乱及Cajal间质细胞缺失

Blocking C-KIT leading disturbance of basic electrical rhythms and loss of interstitial cells of Cajal in murine small intestine

目的观察小鼠小肠在体基本电节律、Cajal间质细胞的分布及阻断C-K IT对其的影响。方法新生的Balb/c小鼠,腹腔内隔天注射C-K IT抗体(ACK2)100μg,共5次,对照组注射生理盐水。于小鼠出生后第10天测定小肠在体基本电节律活动。用免疫组化方法观察小肠Cajal间质细胞的分布。结果对照组小鼠的小肠在体基本电节律活动表现为近似正弦波形,ACK2处理组表现无规律。ACK2处理组小肠基本电节律的频率及幅度为(3.515±1.033)次/m in和(0.045±0.016)mV,均显著低于对照组的(13.997±0.976)次/m in和(0.086±0.018)mV(P<0.01)。对照组Cajal间质细胞主要分布于肌间神经丛区(ICC-MY)及深肌层丛区(ICC-DMP),而ACK2处理组未能明显观察到其分布。结论阻断C-K IT可导致小鼠小肠基本电节律紊乱及Cajal间质细胞缺失。

Objective To investigate the basic electrical rhythms in vivo and distribution of interstitial cells of Cajal （ICC）, and the effect of blockade of C-KIT on them in the Balb/c murine small intestine. Methods New-born murine were intraperitoneally with injected antibody to C-KIT（ ACK2 ） 100 μg every second day, and control groups were injected saline. The basic electrical rhythms in vivo were determined and the distribution of ICC was observed by immunohistochemistry when the murine were 10 days postpartum. Results The basic electrical rhythms in vivo were proximal cosinal waves in control groups and irregular in ACK2 groups. The frequency and the amplitude in ACK2 groups were significantly lower than that in control groups（P 〈0. 01 ）. ICC-MY and ICC-DMP were observed in control groups but the distribution of ICC lost in ACK2 groups. Conclusion The disturbance of basic electrical rhythms and the loss of ICC could be observed when C-KIT was blocked.