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过氧化氢对白癜风患者黑素细胞的氧化损伤 被引量：2

Oxidative damage of H_2O_2 on cultured melanocyte of vitiligo
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摘要 目的探讨过氧化氢(H2O2)对体外培养黑素细胞的影响及氧化应激与白癜风发病的关系。方法培养的黑素细胞中加入H2O2,用MTT法检测黑素细胞增殖,NaOH法检测黑素合成。结果H2O2可以抑制黑素细胞增殖及黑素合成,且具有浓度和时间依赖性。结论氧化应激可以抑制黑素细胞增殖及黑素合成,提示氧化损伤不足以诱发白癜风皮损。 Objective To study the effect of n202 on the cultured melanocytes and the relationship between oxidative stress and vitiligo. Method Melanocytes were cultured with H2O2, the proliferation and melanogenesis of melanocyte were analysed by MTT and NaOH assay. Result H2O2 can inhibite the proliferation and melanogenesis of melanocytes, and the inhibition was concentration and-time-dependent. Conclusion Oxidative stress can inhibite the proliferation and melanogenesis of melanocytes, but oxidative damage can not induce vitiligo.
作者 杨红 王克玉 王奕
机构地区 山东大学齐鲁医院皮肤科
出处 《基础医学与临床》 CSCD 北大核心 2006年第12期1363-1366,共4页 Basic and Clinical Medicine
关键词 过氧化氢 黑素细胞 氧化应激 H2O2 melanocyte oxidative stress
分类号 R758.41 [医药卫生—皮肤病学与性病学]
  • 相关文献

参考文献9

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二级参考文献21

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共引文献31

同被引文献19

  • 1谷梅,柏志全,肖瑞,江振友.过氧化氢对黑素细胞生物学作用的研究[J].岭南皮肤性病科杂志,2006,13(3):188-190. 被引量：5
  • 2徐昱,严淑贤,胡跃.白癜风患者皮损与正常皮肤疱液中抗氧化酶和脂质过氧化物水平比较[J].检验医学,2004,19(4):321-323. 被引量：11
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  • 10Schallreuter KU,Gibbons NC,Zothner C,et al.Hydrogen peroxide-mediated oxidative stress disrupts calcium binding on calmodulin:more evidence for oxidative stress in vitiligo.Biochem Biophys Res Commun 2007;360(1):70-75.

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二级引证文献6

基础医学与临床
2006年 第12期

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