异基因脾细胞诱导大鼠免疫耐受实验研究

The Effects of Immunological Tolerance Induced by Administration of Allogeneic Splenocytes in Rats

目的比较异基因脾细胞经受体门静脉和口服两种途径输注诱导免疫耐受的效果。方法将供体SD大鼠的脾细胞经门静脉或经口服途径输注给受体Wistar大鼠,1周后把SD大鼠的皮肤移植到相应的Wistar大鼠上。观察术后移植皮肤的存活时间,受体外周血淋巴细胞CD25的表达水平和外周血T淋巴细胞亚群,以判断耐受水平。结果移植皮肤的平均存活时间(MST)在门静脉耐受诱导组为15·8±2·1天,口服耐受诱导组为13·0±1·3天,均较对照组长(P<0·01);组间MST比较门静脉耐受诱导组比口服耐受诱导组长(P<0·01);随各组移植皮肤存活时间的延长,外周血淋巴细胞的CD25表达水平呈下降趋势;外周血T淋巴细胞亚群呈现CD4+T细胞下降,CD8+T细胞上升,CD4/CD8比值减少的趋势。结论经门静脉途径输注异基因脾细胞诱导的免疫耐受效果比经口服途径好。

Objective To compare the effects of immunological tolerance induced by administration of allogeneic splenocytes through portal vein and intestinal tract. Methods Administration of the splenocytes from the SD rat to the Wistar rat,through portal vein or intestinal tract. Allogeneic skin transplantation was established a week later. The survival time of the grafts was observed and the immunological states of the recipients were evaluated after skin transplantation. Results The graft mean survival time（MST） in the portal venous tolerance group and the oral tolerance group were 15.8 ± 2. ld and 13.0 ± 1.3d respectively,significantly longer than that in the control group （ P 〈 0. 01 ） ; MST in the portal venous tolerance group was significantly longer than that in the oral tolerance （P 〈 0. 01 ）. Measurement of CD25^＋ T lymphocytes and T lymphocytes subsets showed lower level of transplantation reaction following administration of the allogeneic splenocytes. Conclusions The effects of immunological tolerance induced by administration of allogeneic splenocytes through portal vein were better than that of through intestinal tract.