病毒性脑炎患者NSE和SICAM-1检测临床意义

Detection of Neuron-specific Enolase and Soluble Intercellar Adhesion Molecule-1 in Serum and Cerebrospinal Fluid in the Patients with Viral Encephalitis

目的观察病毒性脑炎患者血清和脑脊液中神经元特异性烯醇化酶(NSE)和可溶性细胞粘附因子-1(sICAM-1)的水平变化并探讨其临床意义。方法采用双抗体夹心法ELISA测定58例病毒性脑炎患者血清和脑脊液标本中NSE和SI-CAM-1水平,并与20例健康人对照比较。结果血清NSE和sICAM-1检测结果正常对照组与治疗前病毒性脑炎急性期组比较有非常显著性差异(P<0·01),与治疗后恢复期脑炎组比较无显著性差异(P>0·05)。脑脊液NSE和sICAM-1检测结果显示:正常对照组与治疗前脑炎急性期组比较有显著性差异(P<0·05和P<0·01),与治疗后恢复期脑炎组比较无显著性差异(P>0·05)。结论NSE和sICAM-1参与了病毒性脑炎感染的病理过程,可作为病毒性脑炎的临床辅助诊断和疗效观察的指标。

Objective To investigate the changes of the neuron - specific enolase（NSE） and soluble intercellar adhesion molecule - 1 （ CAM - 1 ） in serum and cerebrospinal fluid（CSF） in the patients with viral encephhalites （VE）. Methods The levels of NSE and sICAM - 1 in serum and CSF were determined before and after treatment using ELISA in 58 patients with VE and 20 normal persons. Resuits The levels of NSE and sICAM - 1 in serum before treatment were obviously higher than those of control group, and their differences were significant （P 〈 0. 01 ）. The levels of N SE and sICAM -1 in CSF before treatment were obviously higher than those of control group, and their differences were significant （ P 〈 0. 05 and P 〈 0. 01 ）. After treatment the levels of control group, but their differences were not significant（ P 〉 0. 05 ）. Conclusions NSE and slCAM - 1 may contribute to pathologic course in infection of VE and the levels of NSE and sICAM - 1 in serum and CSF may serve as markets of differential diagnosis and clinical significance.