期刊文献+

中介素1-53对油酸致大鼠急性肺损伤的保护作用 被引量:21

The protective effects of intermedin 1-53 on oleic acid induced acute lung injury in rats
原文传递
导出
摘要 目的探讨血管活性肽中介素1-53对油酸致急性肺损伤(ALI)的保护作用及其可能机制。方法将雄性Wistar大鼠24只按随机数字表法分为正常对照组、油酸组和治疗组,每组8只。尾静脉注射油酸(0.2ml/kg)制备大鼠ALI模型,注射前1h及注射后2h各腹腔注射中介素1-53(每次2nmol/kg)。6h后分别行动脉血气分析检查,观察肺组织形态学变化,测定肺湿干质量比值(W/D)、支气管肺泡灌洗液(BALF)中白细胞计数及中性粒细胞分类;测定肺组织髓过氧化物酶活性(MPO)、丙二醛(MDA)和共轭二烯键(C-diene)含量。结果治疗组动脉血氧分压(PaO2)为[(81.7±4.6)mmHg,1mmHg=0.133kPa],肺W/D比值为5.49±0.63,BALF中白细胞计数及中性粒细胞分别为(57.9±7.4)×104/ml、0.718±0.085,与油酸组[(60.8±3.2)mmHg、6.18±0.34、(122.0±16.6)×104/ml、0.878±0.026]比较差异均有统计学意义(q值分别为16.74、3.43、17.23、6.32,P均<0.05、0.01)。结论中介素1-53能明显降低肺组织的脂质过氧化反应,减轻肺组织的损伤程度,对油酸致大鼠ALI具有一定的保护作用。 Objective To investigate the effects of intermedin 1-53 on oleic acid induced acute lung injury (ALl) in rats and its possible mechanisms. Methods Twenty-four male Wistar rats, weighting 280- 300 g,were randomly divided into three groups ( n = 8 ). In the oleie acid group, ALl was induced by injecting oleie acid(0.2 ml/kg) intravenously. In the intervention group, the rats received oleie acid as above, but intraperitoneal injection of intermedin 1-53(2 nmol/kg in 1ml saline) was given 1 h before and 2 h after oleic acid infusion, respectively. The control group was given normal saline (0.2 ml/kg ). The animals were observed for 6 h after intravenous infusion. The histopathologieal changes of lung tissue were studied. The arterial blood gases, lung wet/dry weight ratio (W/D), leukocyte count and neutrophil differentials in bronehoalveolar lavage fluid (BALF) were measured. Contents of intermedin 1-53 in the plasma were detected with radioimmunoassay, myeloperoxidase activity, and malondialdehyde (MDA) and conjugated diene (C-diene) level of lung tissue were also measured. Results The PaO2 was higher and lung wet/dry weight ratio, leukocyte count and neutrophil counts in BALF were significantly lower in the intermedin group [ (81.7±4.6) mm Hg (1 mm Hg=0. 133 kPa) , 5.49 ±0.63, (57.9±7.4)×10^4/ml, 0.718±0. 085, respectively] as compared to the oleie acid group [ (60.8±3.2) mm Hg, 6.18±0.34, ( 122.0±16.6) × 10^4/ml, 0. 878±0.026] , q value was 16.74,3.43,17.23,6.32, respectively, all P 〈 0.05 or 0.01 ). Conclusions Intermedin 1-53 treatment significantly attenuated oleie acid-lnduced lung injury in rats. Intermedin 1-53 exerts pulmo-proteetive effects by inhibiting formation of lipid oxide products.
出处 《中华结核和呼吸杂志》 CAS CSCD 北大核心 2006年第12期808-811,共4页 Chinese Journal of Tuberculosis and Respiratory Diseases
基金 国家自然科学基金资助项目(30471915)
关键词 呼吸窘迫综合征 成人 过氧化 脂质 油酸 中介素1-53 Respiratory distress syndrome, adult Lipid peroxides Oleic acid Intermedin 1-53
  • 相关文献

参考文献10

  • 1Roh J,Chang CL,Bhalla A,et al.Intermedin is a calcitonin/calcitonin gene-related peptide family peptide acting through the calcitonin receptor-like receptor/receptor activity-modifying protein receptor complexes.J Biol Chem,2004,279:7264-7274.
  • 2Poyner DR,Sexton PM,Marshall I,et al.International Union of Pharmacology.XXXⅡ.The mammalian calcitonin gene-related peptides,adrenomedullin,amylin,and calcitonin receptors.Pharmacol Rev,2002,54:233-246.
  • 3Broccard AF,Shapiro RS,Schmitz LL,et al.Influence of prone position on the extent and distribution of lung injury in a high tidal volume oleic acid model of acute respiratory distress syndrome.Crit Care Med,1997,25:16-27.
  • 4罗武生,郭兆贵.用髓过氧化酶法定量测定心肌组织中的中性白细胞[J].中国药理学通报,1990,6(4):264-266. 被引量:126
  • 5Ohkawa H,Ohishi N,Yagi K.Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction.Anal Biochem,1979,95:351-358.
  • 6Recknagel RO,Glende EA Jr.Spectrophotometric detection of lipid conjugated dienes.Methods Enzymol,1984,105:331-337.
  • 7Takei Y,Inoue K,Ogoshi M,et al.Identification of novel adrenomedullin in mammals:a potent cardiovascular and renal regulator.FEBS Lett,2004,556:53-58.
  • 8Yang JH,Jia YX,Pan CS,et al.Effects of intermedin(1-53) on cardiac function and ischemia/reperfusion injury in isolated rat hearts.Biochem Biophys Res Commun,2005,327:713-719.
  • 9Chen L,Kis B,Hashimoto H,et al.Adrenomedullin 2 protects rat cerebral endothelial cells from oxidative damage in vitro.Brain Res,2006,1086:42-49.
  • 10Burak Kandilci H,Gumusel B,Wasserman A,et al.Intermedin/adrenomedullin-2 dilates the rat pulmonary vascular bed:dependence on CGRP receptors and nitric oxide release.Peptides,2006,27:1390-1396.

共引文献125

同被引文献168

引证文献21

二级引证文献40

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部