高转移性人肺腺癌细胞株SPC-A-1BM的建立及其特性分析

Establishment and characterization of a human lung adenocarcinoma cell line SPC-A-1BM with high metastatic potential

目的:建立高转移性人肺腺癌细胞株SPC-A-1BM及其免疫缺陷小鼠转移动物模型。方法:将人肺腺癌细胞株SPC-A-1经免疫缺陷小鼠血道或肺原位接种后形成转移,在放射性核素示踪下找到骨转移病灶,然后切除病变骨组织进行体外培养获得转移性肺癌细胞,用这些癌细胞重复以上循环10次,获得高转移肺癌细胞。采用荧光定量PCR方法检测亲代细胞和高转移细胞的基因表达。结果:获得以骨转移为主,肺、肾上腺、淋巴结等多脏器转移的人肺腺癌转移细胞株(SPC-A-1BM)及其免疫缺陷小鼠动物模型。定量PCR检测显示SPC-A-1BM的上皮生长因子受体(EGFR/HER)家族、血管内皮生长因子(VEGF)家族和3个抗凋亡蛋白的基因较原代细胞均有不同程度的变化。结论:SPC-A-1BM是以骨转移为主的高转移性人肺腺癌细胞株,该细胞株及其转移动物模型为肺癌转移的生物学研究提供了一个良好的技术平台。

Objective：To establish a human lung adenocarcinoma cell line （SPC-A-1BM） with high metastatic potential and its severe combined immunodeficient （SCID） mouse model. Methods： The human lung ad cancer cells SPC-A-1BM were inoculated into the cardiac ventricle or tail vein or implanted orthotopically into the lungs of the SCID mice. The bone meta- static lesions of tumor-bearing mice were imaged with the radionuclide tracing method. The bone lesions were resected and the tumor cells were separated and cultured in vitro. This process was repeated for 10 cycles to obtain a novel high metastatic lung cancer cell subline. The gene expression in both parent and metastatic cells was measured by real-time quantitative polymerase chain reaction. Results：The established SPC-A-1BM cell line had a high metastatic potential in bone, with multiple metastasis in the lung, adrenal gland, lymph node, etc. A significant difference was noticed between parent and metastatic cells in the gene expression of the epidermal growth factor receptor （EGFR） family, the vascular endothelial growth factor （VEGF） family, and 3 anti-apoptotic proteins. Conclusion： SPC-A-1BM cell is a human lung adenocarcinoma cell line with high metastatic potential in bone. The cell line and its metastatic animal model provide a good platform for researching the biological behavior of the metastasis of lung cancer.