Dickkopf(DKK1)在肝癌组织及多种人肿瘤细胞系中的表达分析

Expression of Dickkopf (DKK1) in liver cancer and in multiple human cancer cell lines

目曲：检测dickkopf（DKK1）在肝癌组织及多种恶性肿瘤细胞系中的表达情况。方法：采用酶联免疫吸附实验、real time PCR、RT—PCR及Western blot对DKK1在肝癌组织及多种恶性肿瘤细胞系的表达情况进行检测。结果：肝癌组织检测RT—PCR，10／15肝癌中高表达；Real—time PCR方法检测，8／8肝癌中高表达；Western blot方法检测，5／8肝癌中高表达。酶联免疫吸附实验检测到DKK1在多种恶性肿瘤细胞系的细胞培养液上清液中均有表达，蛋白质表达量波动于5～210ng／mL之间；10种人恶性肿瘤细胞系在mRNA水平和蛋白质水平DKK1的表达均能检测到。结论：DKK1高表达于肝癌组织及广泛表达于多种恶性肿瘤细胞系，提示DKK1可能在肿瘤的发生、发展中起到重要作用。

Objective：To detect the expression of dickkopf （DKK1） in liver cancer and in multiple human cancer cell lines. Methods： The expression of DKK1 in liver cancer and in multiple human cancer cell lines was detected by enzyme-linked immunosorbant assay （ELISA）, reverse transcriptase-polymerase chain reaction （RT-PCR）, real-time PCR, and Western blotting. Results： Semi-quantitative PCR demonstrated that the expression of DKK1 was up-regulated in 10 out of 15 hepatocellular carcinoma （HCC） tissues. Real-time PCR showed the over-expression of DKK1 in 8 out of 8 HCC tissues and Western blotting detected the over-expression of DKK1 in 5 out of 8 HCC tissues. DKK1 protein was detected in the culture medium of all 19 cancer cell lines by ELISA analysis. The concentration of DKK1 protein ranged from 5 to 210 ng/mL. DKK1 could be detected at both mRNA and protein levels in 10 kinds of human cancer cell lines. Conclusion： DKK1 was over-expressed in HCC tissues and widely expressed in multiple human cancer cell lines. DKK1 might play an important role in tumor initiation and development.