2,6-二叔丁基对甲酚致大鼠肺巨噬细胞凋亡的作用

Butylhydroxytoluene induce apoptosis to pulmonary macrophage in rat lung

目的研究2,6-二叔丁基对甲酚(BHT)致大鼠肺巨噬细胞凋亡的作用。方法SD大鼠40只,随机分为BHT高、中、低剂量组(300,600和1 200 mg/kg)和吐温-80溶剂对照和蒸馏水对照,每天灌胃,连续8 d。处死动物,行肺灌流,用荧光染色及彗星试验检测巨噬细胞凋亡。结果肺脏器系数随染毒剂量增加而增高。病理检测见中、高剂量组大鼠肺泡间隔增厚,炎细胞浸润、无纤维化。细胞凋亡检测显示,中、高剂量的BHT具促进肺巨噬细胞凋亡的作用。结论亚急性BHT大剂量染毒可造成大鼠肺、肝等脏器功能性和器质性损伤,BHT可诱发肺巨噬细胞凋亡的产生。中、高剂量组细胞凋亡的百分率分别33.7±3.6和21.2±2.9,与吐温-80(15.6±3.1)和对照(12.3±2.8)组比较,差异均有统计学意义(P<0.05)。

Objective In order to study the apoptosis effect on pulmonary macrophage induced by butylhydroxytoluene （BHT）. Methods 40 SD rats were divided randomly into three different doses groups of BHT and one group as menstruum control and one group as water control. The rats belonging to BHT groups were given separately by the dose of 300, 600 and 1 200 mg/kg （po）, for 8 days.Results At the end of exposure, coefficient of lung was increased with the BHT dose raising. Pulmonary pathology determination showed that there were raised alveolus spacing and interstitial inflammation in middle and high BHT dose groups, but fibrosis were not found. BHT could induce apoptosis to pulmonary macrophage in the perfusate of isolate perfused rat lung as examined by the technology of PI fluorescence stain and comet assay. Conclusion BHT may cause functional and organic damage of lung and liver and it can induce apoptosis to pulmonary macrophagein rat.