摘要
目的建立交联透明质酸钠凝胶中交联剂二乙烯基砜（DVS）残留量的测定方法。方法用高效液相色谱法测定DVS残留量，色谱条件为：色谱柱为C8（4．6mm×250mm），流动相为25mmol／L磷酸二氢钾溶液（pH3．0）-乙腈（90：10），检测波长为210nm。结果DVS在1～50μg／g范围内线性关系良好（r=0．9997），平均回收率98．4％，RSD为1．02％。结论用高效液相色谱法测定交联透明质酸钠凝胶中DVS残留量，方法准确、可靠，可用于产品质量控制。
Objective To analyse divinylsulfone from cross- linked hyaluronan gels. Methods The content of divinylsulfone in gels was determined by HPLC with 25mM potassium dihydrogen phosphate solution （pH3.0） and acetonitrile （90：10）as mobile phase at a flow rate of 1 ml/min.UV detection wavelength was set at 210 nm.A HiQ sil C8 column was used at 30℃ in analysis.Results A good linearity was obtained in the range of 1 - 50μg/g （ r = 0.999 7） for DVS.The average recovery of DVS was 98.4% with RSD of 1.02%. Conclusion The analysis method for DVS is accurate and reliable,and can be used for detect content of DVS in DVS- HA gels.
出处
《上海生物医学工程》
2006年第4期223-225,共3页
Shanghai Journal of Biomedical Engineering
