阿仑膦酸钠不同给药时间间隔对磨损颗粒诱导的假体周围骨溶解影响

Effects of alendronate admisnistrated by different intervals on periprosthetical osteolysis induced by wear particles

目的观察阿仑膦酸钠不同给药时间间隔对磨损颗粒诱导假体周围骨溶解的影响,为临床应用阿仑膦酸钠预防人工关节假体周围骨溶解提供理论依据。方法雄性SD大鼠12只,经膝关节将钛合金假体及混合磨损颗粒植入胫骨近端(双侧),随机分成实验Ⅰ组和实验Ⅱ组,每组6只,术后分别每日1次空腹阿仑膦酸钠(0.1 mg/kg)灌胃和每周1次空腹阿仑膦酸钠(0.7 mg/kg)灌胃,持续6周。术后12周处死取材,进行组织学观察及组织形态计量学测定。结果实验Ⅰ组和实验Ⅱ组假体柄周围纤维界膜均较薄,细胞成分少,假体周围新生骨与假体间可见有直接接触,对假体支撑作用良好。假体周围界膜厚度及面积的形态计量学检测发现,两组间差异无显著性。结论阿仑膦酸钠预防磨损颗粒诱导的假体周围骨溶解时,每周给药1次与每日给药1次可产生同样的效果。

Objective To observe the effects of Alendronate administrated by different intervals on the periprosthetical osteolysis induced by wear particles and to provide theory evidence for clinic use of Alendronate for prevention of periprosthetical osteolysis. Methods Specially designed implants made of Ti alloy and mixed wear particles were placed into the proximal part of bilateral tibias of 12 SD rats. Then the rats were divided into the experimental Ⅰ group （6 rats） and the experimental Ⅱ group（6 rats） randomly. In the experimental Ⅰ , n groups, alendronate, 0. 1 mg/kg/day and 0. 7 mg/kg/week, was respectively perfused into the empty stomach daily for 6 weeks. At 12 week postoperatively, these rats were sacrificed with overdose anesthesia and all of the tibias were harvested. Then histology and histomorphometry studies were carried out. Results The histological analysis showed that there were fibrous membrane and the newly formed bone around the implant in two experimental groups. The fibrous membrane was rare and thin with few cells. It was seen that the newly formed bone could support the prosthesis. Thickness and area of the fibrous membrane around the implant tested by the histomorphometry showed that it was insignificant different between the experimental I group and the experimental Ⅱ group in statistics. Conclusions When used to prevent periprosthetical osteolysis induced by wear particles of artificial joint, the effect with once-weekly alendronate is the same as that with once-daily.