幽门螺杆菌粪便抗原检测对诊断儿童幽门螺杆菌感染的价值

Diagnostic Significance of Detecting Helicobacter Pylori Antigen in Stools for Child Helicobacter Pylori Infection

目的评价酶联免疫法检测幽门螺杆菌粪便抗原(HpAg)在诊断儿童幽门螺杆菌(Hp)感染及治疗后复查的价值。方法采用快速尿素酶试验,组织切片染色法,酶联免疫分析法,细菌分离培养Hp。结果ELISA检测粪便HpAg阳性率(85.2%)与其他3种方法差异无显著性(P>0.05)。HpAg的敏感度为98.1%,特异性为84.2%,阳性预测值为97.1%,阴性预测值为88.9%,总有效率为95.1%。结论HpAg具有简便、经济、无创伤性等特点,可用于儿童Hp感染的诊断和观察治疗后Hp的转阴情况。

Objective To evaluate the diagnostic value of detecting helicobacter pylori antigen （HpAg） by enzyme-linked immunosorbent assay （ELISA） in stools for child HpAg infection and its significance of re-examination after treatment. Methods We utilized rapid urokinase test, histological slice stain, ELISA, and separation and culture of HpAg. Results The positive rate of ELISA for stool HpAg （ 85.2% ） was not significantly different from that of the other three methods. Furthermore, its HpAg sensi- tivity, specificity, positive prognostic value, negative prognostic value and total efficacy were 98.1% , 84.2% , 97.1%, 88.9% and 95.1% , respectively. Conclusion The detection of HpAg in stools is characteristic of convenience, cheapness and wound- lessness. It can be applied to the diagnosis of HpAg infection and to the recognition of its conversion to negative after treatment in children.