雌二醇诱导大鼠隐睾动物模型建立

Estradiol-induced Cryptorchidism : a Rat Model

目的为研究隐睾发生及其损害机制建立合理动物模型。方法对新生雄性SD大鼠,在出生后第3～30 d,分别每天给予皮下注射1．0μg17-β雌二醇(49只,干预组)和等剂量丙二醇(10只,对照组)。在第30～35 d观察隐睾发生情况。结果干预组隐睾的发生率为93．9％,其中单侧隐睾的发生率为24．5％,双侧隐睾的发生率为69．4％;丙二醇组隐睾发生率为0。结论通过外源性雌二醇个体干预雄性SD大鼠的方法可以获得理想的隐睾动物模型。

Objective To study eryptorchidism occurrence and testes impairment mechanisms,we developed an animal model with 17-15 estradiol. Methods 49 Male Sprague Dawley rats were injected subcutaneously 1.01μg 17-β estradiol from the third day of life to the 30th day. Control group of 10 male SD rats were injected same volume of propylene glycol. The cryptorchidism rats were judged at above 36℃ environmental temperature at day 30 postpartum （dpp） to 35dpp. Results The incidence rate of cryptrochidism was 93.9%, the unilateral 24.5% and bilateral 69.4% respectively. However, in the control group no cryptrochidism was found.Conclusion The animal model establishment of cryptorchidism induced by exogenous estradiol could obtain an ideal modality in cryptorchidism.