出国移民中丙型肝炎病毒感染检测结果分析

The Analysis of Hepatitis C Virus Infection among Chinese Emigration

〔目的〕探索简便而准确的丙型肝炎血清学诊断方法,并了解一般人群中丙型肝炎病毒感染的状况。〔方法〕以酶联免疫吸附试验(ELISA)作为主要检测方法,用金标法复核,并与HCV-RNA的检测进行对比;同时对2004年7月￣2006年6月的26366份血样抗-HCV检测的结果进行分析。〔结果〕用ELISA法为主,结合金标法来判断抗-HCV的检测结果,与HCV核酸检测的方法符合率为96.0%;在出国移民中丙型肝炎病毒感染率为0.44%,且男性高于女性,其中有64.10%的人丙氯酸转氨酶(ALT)超过正常值范围。〔结论〕用ELISA与金标结合的方法判断抗-HCV检测结果,可避免单纯使用ELISA方法假阳性较高的现象;有2/3的HCV感染者中ALT超过正常值范围。

Objective To probe a simple and precise method for Hepatitis C serological diagnosis,and to understand the sifuation of Hepatitis C Virus Infection. Methods 26 366 serum samples from July 2004 to June 2006 were detected anti-HCV by ELISA and confirmed by Golden Method （Colloidal gold method）, comparing with HCV-RNA by PCR. Anti-HCV ELISA （＋） samples was confirmed by detecting HCV-RNA. Results Compared with HCV-RNA by PCR, the coincidence of anti-HCV detected by ELISA and Golden Method was 96.0%.The infection rate of Hepatitis C Virus was 0.44% among emigration,and the infection rate for males was higher than that of females＇ .While,the abnormal serum ALT was 64.10%. Conclusions Combine ELISA and Golden Method to judge the detection of anti-HCV can avoid the phenomenon of higher false positive rate caused by using ELISA only.Meanwhile,the abnormal serum ALT was found in almost two thirds of HCV infectors.