原发性胆汁性肝硬化血清抗线粒体抗体亚型的检测及诊断价值

The clinical presentation of primary biliary cirrhosis and the diagnostic value of anti-mitochondrial antibody subtype

目的探讨抗线粒体亚型(AMA亚型)对原发性胆汁性肝硬化(PBC)的诊断价值。方法应用酶免疫斑点法检测血清中AMA-M2、M4、M9亚型。8例经临床诊断为PBC患者,47例非PBC肝胆病患者。结果8例PBC患者M2均阳性、但M4及M9均阴性;47例非PBC肝胆病患者M2、M4、M9均阴性。4例PBC者肝活检Ⅰ期及Ⅳ期各1例、2例为Ⅱ期。结论血清AMA-M2抗体检测可作为临床诊断PBC的重要血清免疫学诊断指标。

Objective This is a study on the dianostic value of anti-mitochondrial antibody subtype （AMA-M2.M4 and M9） in the patients of primary biliary cirrhosis （PBC）.Methods The enzyme immunc blot assay were used for the detection of AMA-M2, M4 and M9 antibodies in 8 PBC patients diagnosed by clinical presentation and 47 patients with other hepato-biliary discases as control Results All the 8 PBC patients diagnosed by clinical presentation were found to be M2 antibody positive while the M4,M9 were all negative. The 47 patients with other hepato-biliary diseases were all negative in AMA- M2, M4 and M9 antibedies. In the 4 of the 6 PBC patients who lind received liver biopsy 2 patients were found to be in stage Ⅱ of PBC,one by one patient in stage Ⅰ and Ⅳ. Conclusion AMA-M2 antibody detection is an important and sencetive seroimmunological marker for the diagnosis of PBC.