甲胎蛋白对树突状细胞表面分子及增殖的影响

Effect of AFP on The Phenotypes and Proliferation of Dendritic Cell

目的研究甲胎蛋白对树突状细胞免疫功能的影响。方法用流式细胞仪分析体外培养扩增的人外周血树突状细胞表面分子的表达及功能变化。MTT法分析树突状细胞增殖情况。结果甲胎蛋白可抑制人外周来源树突状细胞增殖,下调DC共刺激分子CD1a、CD83、CD80、CD86、CD11c的表达,甲胎蛋白的免疫抑制效应与其浓度有关。结论甲胎蛋白下调抑制树突状细胞生长及其免疫功能。

Objective To study the effect of AFP on the immunal function and proliferation of dendritic cell. Methods The proliferation of DC was tested by MTr. The expression of immunal phenotypes of cultured human dendritic cell were examed by using FAGs. Results The proliferation of dendritic cell is suppressed by AFP. The immunal phenotypes （CD1a, CD83 CD80, CD86, CD11c）were down-regulated markedly by AFP. Conclusion AFP can suppress the proliferstion and immunal function of dendritic cell.