P选择素在大鼠皮肤损伤愈合过程中的免疫组织化学表达

The immunohistochemical expression of P-selectin in the healing process of incised skin wounds of rats

目的探讨P选择素的表达变化与损伤时间的关系。方法应用免疫组织化学技术,对实验大鼠不同损伤时间的生前伤(5 min-7 d)皮肤组织中P选择素的表达变化进行观察,以大鼠死后伤组作为对照组。结果在生前损伤组,最早至伤后10 min,P选择素即在血管内皮呈阳性表达,持续至伤后7 h,且P选择素随损伤时间变化呈规律性表达。在死后伤组未见上述改变。结论P选择素可作为法医学皮肤损伤时间判定的有效指标。

Objective To investigate the ilnmunohistochemieal exprssion of IL-8 and VEGF in wound healing process for possible application in the determination of incised wound age.Methods An immunohistochemical study on the expression of P-sclectin was performed on rats vital akin incised wounds（5 min-7 d）,and the postmortem incises were used as control.Results In vital skin incisions, the positive stain of P-selectin was detected as early as 10 rain after incised and lasted fill 7 h in the vascular endothelium, the ratios of P-selectin positive vascular endothelial cells changed regularly according to different ,round time.There were no changes of P- selectin in the postmortem incised group. Conclusion P-aelectin is considered potentially useful markers for wound age determination in forensic practice.