摘要
建立测定人血浆中奥美拉唑浓度的高效液相色谱法,并应用于人体药代动力学研究。血浆样品中加入内标非那西丁后用乙酸乙酯提取,色谱柱为Zorbax SB—C18柱,流动相为0.05mol·L^-1乙酸铵溶液(用氨水调至pH7.0)-乙腈(68:32),流速为1.0ml·min^-1,紫外检测波长302nm。血浆中内源性物质对样品测定无干扰。本方法线性范围为0.01~2μg·ml^-1(r=0.9997),最低定量浓度为0.01μg·ml^-1,提取回收率大于80%,方法回收率为98.6%~103.1%,日内、日间RSD均小于6%。本法简便、准确,适用于奥美拉唑药代动力学的研究。
A HPLC method for the determination of omeprazole in human plasma was established, and was used on the research of pharmacokinetics in healthy volunteers. After addition of phenacetin ( internal standard), plasma samples was extracted with ethyl acetate and determined. A Zorbax SB - C18 column was used with the mobile phase of 0. 05mol·L^-1 ammonium acetate solution adjusted to pH 7.0 with concentrated ammonia) and acetonitrile (68: 32) with a flow rate of 1.0ml ·mol-1 The detection wavelength was at 302nm. The blank plasma did not interfere with the determination. The calibranon range was 0. 01 -2 μg·ml^-1 (r =0. 9997 ), and the quantitative limit was 0. 01 μg·ml^-1. The extraction recovery was more than 80%, and the method recovery was 98.6% - 103. 1%. RSD of within - day and between - day were less than 6%. The method is simple and accurate, and suitable for the pharmacokinetic study of omeprazole.
出处
《中国药事》
CAS
2006年第12期759-761,共3页
Chinese Pharmaceutical Affairs