可用于负载营养成分的脂质纳米粒的制备及其应用

Preparation and application of lipid nanoparticles carrying functional nutriments

采用自制的相图检测装置研究了一种特殊微乳液体系的相图,确定其相行为,基于相行为制备了脂质纳米粒并成功负载了VE。这种微乳液体系的特殊之处在于其油相材料在室温下为固体,必须在其熔点以上变为熔融液相才可用于制备微乳液。研究中的油相材料为单硬脂酸甘油酯(GMS)和油酸(OA),复配乳化剂为S-40和F-68。相行为的研究确定了该体系O/W微乳液的区域,为脂质纳米粒的制备提供了配方基础。基于相行为研究制备了平均粒径为10nm的GMS纳米粒,得到外观与水溶液完全相同的GMS纳米粒水分散体系。采用该纳米粒载体体系可以负载VE。在前述研究基础上,利用自制的实验室级80L制备装置成功实现了50L脂质纳米粒水分散体系的小试生产,为该技术的真正工业应用奠定了良好基础。

The self-made phase graphs detector was used in this paper to study the phase graph ef one kind of special microemulsion, and define it＇s phase behavior. Then basing on the phase behavior the lipid nanoparticles ware prepared and the vitamin E was successfully carried in the nanoparticles. The lipid materials of the microemulsion are solid in room-temperature, so they must be heatec to be melting liquid phase to prepare the microemulsion. The lipid materials of this study are glycerol monostearate（GMS） and oleic acid（OA）, and the emulsifying agent are polyoxyl [40] Stearate（S-40） and PoLoxamer 188（F-68）. The study of the phase behavior defines the O/W region of the microemulsion and supplies formulations of the production of lipid nanoparticles. Basing on the study of the phase behavior, the GMS nanoparticles about 10nm were prepared, and the GMS nanoparticles-water disperse system achieved has the same external appearance with water. This nanoparticles carrier system can be used for carrying the vitamin E. On the above study, the small scale production of 50L nannopaticles-water disperse system was successfully realized in the self-made laboratoryscale 80L preparation device. The success gives a good ground for the application of this technology.